Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7570 | 3' | -44.2 | NC_001962.1 | + | 106533 | 0.67 | 0.999985 |
Target: 5'- aAGAcgUCgUACUCGUAAAGuCCcGUgUCGu -3' miRNA: 3'- -UCUuaAG-AUGAGCAUUUC-GGuCA-AGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 64903 | 0.67 | 0.999971 |
Target: 5'- uAGAAUUCUACUUGUAAcGCacgauuaAGUaugaaUCAu -3' miRNA: 3'- -UCUUAAGAUGAGCAUUuCGg------UCA-----AGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 86518 | 0.68 | 0.999869 |
Target: 5'- uAGAAUUCUACUUGUAAAacaaauuGUCGGaugaugUCAu -3' miRNA: 3'- -UCUUAAGAUGAGCAUUU-------CGGUCa-----AGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 107196 | 0.69 | 0.999832 |
Target: 5'- uAGAAUUCUGCcCGUAAAuCaAGUUCGa -3' miRNA: 3'- -UCUUAAGAUGaGCAUUUcGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 107088 | 0.72 | 0.99646 |
Target: 5'- uAGAAUUCUACUCGUAAAacgaauUCGGUUa- -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUc-----GGUCAAgu -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 122004 | 0.81 | 0.776311 |
Target: 5'- uGAucGUUCUACUCGU-AAGCaCAGUUCAa -3' miRNA: 3'- uCU--UAAGAUGAGCAuUUCG-GUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 90110 | 0.84 | 0.644459 |
Target: 5'- -aAAUUCUACUCGUAAAGCgAGUUUg -3' miRNA: 3'- ucUUAAGAUGAGCAUUUCGgUCAAGu -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 107655 | 0.85 | 0.576562 |
Target: 5'- uAGAAUUUUACUCGUAAAGCgAGUUg- -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGgUCAAgu -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 89615 | 0.87 | 0.510376 |
Target: 5'- uAGAAUUaUACUCGUAAAGCgAGUUCAa -3' miRNA: 3'- -UCUUAAgAUGAGCAUUUCGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 22626 | 0.89 | 0.417815 |
Target: 5'- uGAAUUCUACUCGUAAAGCgAGUUa- -3' miRNA: 3'- uCUUAAGAUGAGCAUUUCGgUCAAgu -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 23423 | 0.95 | 0.20956 |
Target: 5'- uAGAAUUUUACUCGUAAAGCgAGUUCAg -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 23286 | 0.95 | 0.20956 |
Target: 5'- uAGAAUUUUACUCGUAAAGCgAGUUCAg -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 23174 | 0.96 | 0.187681 |
Target: 5'- uAGAAUUCUACUCGUAAAGCgAGUUUAa -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 22734 | 0.97 | 0.167838 |
Target: 5'- uAGAAUUaUACUCGUAAAGCCAGUUCAg -3' miRNA: 3'- -UCUUAAgAUGAGCAUUUCGGUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 22914 | 0.97 | 0.167838 |
Target: 5'- uAGAAUUaUACUCGUAAAGCCAGUUCAg -3' miRNA: 3'- -UCUUAAgAUGAGCAUUUCGGUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 107461 | 1 | 0.109221 |
Target: 5'- uAGAA-UCUACUCGUAAAGCCAGUUCAg -3' miRNA: 3'- -UCUUaAGAUGAGCAUUUCGGUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 22806 | 1.01 | 0.100065 |
Target: 5'- uAGAAUUCUACUCGUAAAGCgAGUUCAg -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGgUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 22986 | 1.11 | 0.026761 |
Target: 5'- uAGAAUUCUACUCGUAAAGCCAGUUCAg -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGGUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 23085 | 1.11 | 0.026761 |
Target: 5'- uAGAAUUCUACUCGUAAAGCCAGUUCAg -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGGUCAAGU- -5' |
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7570 | 3' | -44.2 | NC_001962.1 | + | 106998 | 1.11 | 0.026761 |
Target: 5'- uAGAAUUCUACUCGUAAAGCCAGUUCAa -3' miRNA: 3'- -UCUUAAGAUGAGCAUUUCGGUCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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