Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7571 | 5' | -45.4 | NC_001962.1 | + | 77859 | 0.66 | 0.999956 |
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Target: 5'- ---cGGGUcgcccugcUUUGCCAcgcuaucuggaGCAUGGUGCUGg -3' miRNA: 3'- cuaaUCUA--------AAAUGGU-----------UGUACCGCGGC- -5' |
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| 7571 | 5' | -45.4 | NC_001962.1 | + | 94117 | 0.66 | 0.999914 |
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Target: 5'- -uUUGGGUUaaugcgucuauuugUUGCCcuuggguCAUGGCGCCu -3' miRNA: 3'- cuAAUCUAA--------------AAUGGuu-----GUACCGCGGc -5' |
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| 7571 | 5' | -45.4 | NC_001962.1 | + | 17180 | 0.69 | 0.998249 |
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Target: 5'- --gUGGugaggACCAGCAgcUGGUGCCGu -3' miRNA: 3'- cuaAUCuaaaaUGGUUGU--ACCGCGGC- -5' |
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| 7571 | 5' | -45.4 | NC_001962.1 | + | 34387 | 0.71 | 0.993067 |
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Target: 5'- -cUUGGcgUacACCAAaAUGGCGCCGg -3' miRNA: 3'- cuAAUCuaAaaUGGUUgUACCGCGGC- -5' |
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| 7571 | 5' | -45.4 | NC_001962.1 | + | 49628 | 0.72 | 0.987719 |
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Target: 5'- cGUUAcGA-UUUGCCGGCugcGGCGCCGa -3' miRNA: 3'- cUAAU-CUaAAAUGGUUGua-CCGCGGC- -5' |
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| 7571 | 5' | -45.4 | NC_001962.1 | + | 120075 | 1.1 | 0.022737 |
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Target: 5'- aGAUUAGAUUUUACCAACAUGGCGCCGc -3' miRNA: 3'- -CUAAUCUAAAAUGGUUGUACCGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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