Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7573 | 3' | -54.7 | NC_001962.1 | + | 75288 | 0.66 | 0.932931 |
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Target: 5'- cGCGU--UCCagaaAACCGCGCAUCG-UACc -3' miRNA: 3'- -CGCAgaAGGg---UUGGUGCGUGGCuGUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 12680 | 0.66 | 0.932931 |
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Target: 5'- cGUGUCg--CCGGCCaACGUGCCGAgAUu -3' miRNA: 3'- -CGCAGaagGGUUGG-UGCGUGGCUgUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 18270 | 0.66 | 0.92769 |
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Target: 5'- gGCGUCUUCguuaauaugCCAAUCACGUACgCaACAa -3' miRNA: 3'- -CGCAGAAG---------GGUUGGUGCGUG-GcUGUg -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 47312 | 0.66 | 0.922203 |
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Target: 5'- gGCGauaacaUUCCCGacGCCGCGUGCCG-CAa -3' miRNA: 3'- -CGCag----AAGGGU--UGGUGCGUGGCuGUg -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 20158 | 0.66 | 0.916469 |
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Target: 5'- uGCGUUUaacgcCCCGcCCGCGUACUacaucaGACACa -3' miRNA: 3'- -CGCAGAa----GGGUuGGUGCGUGG------CUGUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 78290 | 0.67 | 0.904268 |
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Target: 5'- aCGUCgcaCaCGGCCAgGCugUGGCACa -3' miRNA: 3'- cGCAGaagG-GUUGGUgCGugGCUGUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 81472 | 0.67 | 0.902355 |
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Target: 5'- aGCGUCcgCCCAuccuacGCCGCGUcCCGuaauaaauuuguuuGCGCg -3' miRNA: 3'- -CGCAGaaGGGU------UGGUGCGuGGC--------------UGUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 31118 | 0.68 | 0.829489 |
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Target: 5'- cGUGUUUUCgCuuUCACGCGCUGcCACa -3' miRNA: 3'- -CGCAGAAGgGuuGGUGCGUGGCuGUG- -5' |
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| 7573 | 3' | -54.7 | NC_001962.1 | + | 49630 | 0.7 | 0.736508 |
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Target: 5'- aGCGUUacgauUUgCCGGCUGCgGCGCCGACAa -3' miRNA: 3'- -CGCAG-----AAgGGUUGGUG-CGUGGCUGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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