miRNA display CGI

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ID	Location	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
7574	3'	-52.2	NC_001962.1	+	34202	0.66	0.971807
	Target:  5'- --gCCGAUU--CCaaAugCGCCGCGCc -3' miRNA:   3'- uaaGGUUAAauGGggUugGCGGCGCG- -5'
7574	3'	-52.2	NC_001962.1	+	61249	0.66	0.965511
	Target:  5'- ---gCAAUUUuuauuacaacACCCCu-CCGCCGcCGCu -3' miRNA:   3'- uaagGUUAAA----------UGGGGuuGGCGGC-GCG- -5'
7574	3'	-52.2	NC_001962.1	+	58987	0.67	0.948329
	Target:  5'- --cCCGAauacaaaauuCCCCGcaugguuauGCUGCCGCGCg -3' miRNA:   3'- uaaGGUUaaau------GGGGU---------UGGCGGCGCG- -5'
7574	3'	-52.2	NC_001962.1	+	31531	0.67	0.940882
	Target:  5'- uUUCCGccuauuaaGUUUAaCCCGacGCCGCCGCa- -3' miRNA:   3'- uAAGGU--------UAAAUgGGGU--UGGCGGCGcg -5'
7574	3'	-52.2	NC_001962.1	+	128038	0.68	0.925132
	Target:  5'- cUUCCug--UACau--ACCGCCGCGCg -3' miRNA:   3'- uAAGGuuaaAUGggguUGGCGGCGCG- -5'
7574	3'	-52.2	NC_001962.1	+	3066	0.69	0.871806
	Target:  5'- uUUCCGGacuaaaaUACUCCAacguGCCGUCGUGCa -3' miRNA:   3'- uAAGGUUaa-----AUGGGGU----UGGCGGCGCG- -5'
7574	3'	-52.2	NC_001962.1	+	77160	0.7	0.830741
	Target:  5'- --aCCAAUUUugCagcuCCGCCGCGUu -3' miRNA:   3'- uaaGGUUAAAugGgguuGGCGGCGCG- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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