Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7575 | 5' | -56.1 | NC_001962.1 | + | 93114 | 0.66 | 0.873575 |
Target: 5'- -cGGaUCGAAGCgcuguugauaucguCGAUACGGCCgugaGGCGCg -3' miRNA: 3'- uaUC-GGUUUUG--------------GCUGUGCCGG----CCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 57262 | 0.66 | 0.85283 |
Target: 5'- ---aCCGAacaAACCGACGC-GCCGGCa- -3' miRNA: 3'- uaucGGUU---UUGGCUGUGcCGGCCGcg -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 2042 | 0.66 | 0.85283 |
Target: 5'- -cAGCCAG---CGGCGCcugcGCCGGUGCc -3' miRNA: 3'- uaUCGGUUuugGCUGUGc---CGGCCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 21330 | 0.66 | 0.844758 |
Target: 5'- cUGGCCA--ACCG-CAUGGCgGacauuGCGCa -3' miRNA: 3'- uAUCGGUuuUGGCuGUGCCGgC-----CGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 78209 | 0.67 | 0.836488 |
Target: 5'- uUGGCCAGcugcGCCGuuuCGCGGC--GCGCg -3' miRNA: 3'- uAUCGGUUu---UGGCu--GUGCCGgcCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 99317 | 0.68 | 0.754432 |
Target: 5'- -aAGCC---GCCGuuACaGCCGGCGUc -3' miRNA: 3'- uaUCGGuuuUGGCugUGcCGGCCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 122467 | 0.68 | 0.754432 |
Target: 5'- uAUGGCguAGACCgGugGCGGC-GGCGa -3' miRNA: 3'- -UAUCGguUUUGG-CugUGCCGgCCGCg -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 98672 | 0.69 | 0.714665 |
Target: 5'- --cGCCGGuGAUCGAUugGGCCGaacgcaauuauGCGCu -3' miRNA: 3'- uauCGGUU-UUGGCUGugCCGGC-----------CGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 71389 | 0.69 | 0.704517 |
Target: 5'- --cGCUAAcguacaaCGACGCuguGGCCGGCGCc -3' miRNA: 3'- uauCGGUUuug----GCUGUG---CCGGCCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 98383 | 0.69 | 0.704517 |
Target: 5'- uUGGUCAAAaucgcagacACCGACgGCGGUCaucuGGCGCc -3' miRNA: 3'- uAUCGGUUU---------UGGCUG-UGCCGG----CCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 36407 | 0.7 | 0.673734 |
Target: 5'- --cGCUAGAcgacggacuuguGCUGuACAUGGCCGGUGUg -3' miRNA: 3'- uauCGGUUU------------UGGC-UGUGCCGGCCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 61062 | 0.7 | 0.621844 |
Target: 5'- -gAGCaaaGAACCGACGCucgacGCCGGCGa -3' miRNA: 3'- uaUCGgu-UUUGGCUGUGc----CGGCCGCg -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 81425 | 0.7 | 0.621844 |
Target: 5'- -gAGCCAAcGCCGuCGC-GCCuGCGCa -3' miRNA: 3'- uaUCGGUUuUGGCuGUGcCGGcCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 77206 | 0.72 | 0.549729 |
Target: 5'- -aGGCuCAAcGCCGACGCGGUgcacUGGgGCa -3' miRNA: 3'- uaUCG-GUUuUGGCUGUGCCG----GCCgCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 8288 | 0.73 | 0.465138 |
Target: 5'- --cGUCAAacuguucacagaaccGACCGGCACGGCaagcuGGCGCu -3' miRNA: 3'- uauCGGUU---------------UUGGCUGUGCCGg----CCGCG- -5' |
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7575 | 5' | -56.1 | NC_001962.1 | + | 60325 | 0.77 | 0.282694 |
Target: 5'- cAUGGCCGAGcuGgCGACGCGaGCCGGCaaGCa -3' miRNA: 3'- -UAUCGGUUU--UgGCUGUGC-CGGCCG--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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