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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7582 | 3' | -48.2 | NC_001962.1 | + | 856 | 0.66 | 0.999087 |
Target: 5'- cGGACCaGUgaaCAgagGCGCGUcuggUGCAa -3' miRNA: 3'- -CCUGGgCAaaaGUaaaCGCGCA----ACGU- -5' |
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7582 | 3' | -48.2 | NC_001962.1 | + | 127010 | 0.67 | 0.998144 |
Target: 5'- -cGCCCGacgaUCAUucgguguacagauacUUGUGCGUUGCGu -3' miRNA: 3'- ccUGGGCaaa-AGUA---------------AACGCGCAACGU- -5' |
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7582 | 3' | -48.2 | NC_001962.1 | + | 97932 | 0.67 | 0.997141 |
Target: 5'- cGGACCCGUUcuauUUCAUgcaCGaCGUUGa- -3' miRNA: 3'- -CCUGGGCAA----AAGUAaacGC-GCAACgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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