Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7582 | 5' | -55.1 | NC_001962.1 | + | 11624 | 0.66 | 0.89236 |
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Target: 5'- aGGCAGAAauagcagcaGCGUgGCUGCCGuaacGCAc -3' miRNA: 3'- -CCGUCUUa--------CGCGgCGGCGGUuuu-CGU- -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 27125 | 0.66 | 0.89236 |
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Target: 5'- aGCGcGAAcGCGcCCGCCGCCGAc---- -3' miRNA: 3'- cCGU-CUUaCGC-GGCGGCGGUUuucgu -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 67601 | 0.66 | 0.89236 |
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Target: 5'- aGGCGGAG-GUGuaGCCGCCuaaaaauGGAGUu -3' miRNA: 3'- -CCGUCUUaCGCggCGGCGGu------UUUCGu -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 81506 | 0.67 | 0.838738 |
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Target: 5'- cGGCGGGAaacgcggGCGUCGUCGUCGc-AGCGc -3' miRNA: 3'- -CCGUCUUa------CGCGGCGGCGGUuuUCGU- -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 41223 | 0.68 | 0.821528 |
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Target: 5'- cGGCGGGA--UGCCGCCGUaauugaGAucGCAg -3' miRNA: 3'- -CCGUCUUacGCGGCGGCGg-----UUuuCGU- -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 56025 | 0.68 | 0.794373 |
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Target: 5'- cGGCGGGcgGCGCCGaagguuuaaCGCUuaauugcgGAGGGCu -3' miRNA: 3'- -CCGUCUuaCGCGGCg--------GCGG--------UUUUCGu -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 21241 | 0.69 | 0.745159 |
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Target: 5'- aGGCGGAgaaaaguAUGCGUuucUGCgCGCgCAAAAGCGc -3' miRNA: 3'- -CCGUCU-------UACGCG---GCG-GCG-GUUUUCGU- -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 41358 | 0.72 | 0.549074 |
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Target: 5'- cGCAG-AUGCGCCGCCGgCuucugauuGGCGc -3' miRNA: 3'- cCGUCuUACGCGGCGGCgGuuu-----UCGU- -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 121906 | 0.74 | 0.459779 |
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Target: 5'- aGCAGca-GCaGCCGCCGCCAcAGGCu -3' miRNA: 3'- cCGUCuuaCG-CGGCGGCGGUuUUCGu -5' |
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| 7582 | 5' | -55.1 | NC_001962.1 | + | 121744 | 0.76 | 0.378791 |
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Target: 5'- cGCAGA---CGUCGCCGUCAGGAGCGg -3' miRNA: 3'- cCGUCUuacGCGGCGGCGGUUUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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