Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7583 | 3' | -53.2 | NC_001962.1 | + | 101771 | 0.66 | 0.937304 |
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Target: 5'- gUUGGuccacACggucgAACCGCCGuuGCUAAu- -3' miRNA: 3'- aAACCu----UGa----UUGGCGGCggCGGUUuu -5' |
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| 7583 | 3' | -53.2 | NC_001962.1 | + | 111748 | 0.66 | 0.93206 |
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Target: 5'- gUUGGAcgauuCUGACCGCauagacauaUGCUGUCAAAAa -3' miRNA: 3'- aAACCUu----GAUUGGCG---------GCGGCGGUUUU- -5' |
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| 7583 | 3' | -53.2 | NC_001962.1 | + | 103422 | 0.66 | 0.914723 |
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Target: 5'- ---------cGCCGCCGCCGCCGc-- -3' miRNA: 3'- aaaccuugauUGGCGGCGGCGGUuuu -5' |
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| 7583 | 3' | -53.2 | NC_001962.1 | + | 52945 | 0.67 | 0.901835 |
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Target: 5'- gUUUGGuugaacGCUGGCCGCUGUuguucgCGCCAAc- -3' miRNA: 3'- -AAACCu-----UGAUUGGCGGCG------GCGGUUuu -5' |
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| 7583 | 3' | -53.2 | NC_001962.1 | + | 679 | 0.7 | 0.73364 |
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Target: 5'- -gUGGAugUucugauugggcAGCCGCCGCCcuuuuuaGCCAGAc -3' miRNA: 3'- aaACCUugA-----------UUGGCGGCGG-------CGGUUUu -5' |
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| 7583 | 3' | -53.2 | NC_001962.1 | + | 27128 | 0.74 | 0.542096 |
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Target: 5'- --cGGAGCgcgAACgCGcCCGCCGCCGAc- -3' miRNA: 3'- aaaCCUUGa--UUG-GC-GGCGGCGGUUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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