Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7583 | 5' | -48.2 | NC_001962.1 | + | 28099 | 0.66 | 0.998791 |
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Target: 5'- ------cGGCGuuaACGGCGGUaUCaGACCa -3' miRNA: 3'- aaaauaaUCGU---UGCCGCCA-AG-CUGGc -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 2549 | 0.66 | 0.998791 |
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Target: 5'- -----gUAGCAuuugugGCGGUGGUggUGGCCa -3' miRNA: 3'- aaaauaAUCGU------UGCCGCCAa-GCUGGc -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 111465 | 0.67 | 0.997423 |
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Target: 5'- ----cUUAGCAccacCGGCGGUUCGuuCu -3' miRNA: 3'- aaaauAAUCGUu---GCCGCCAAGCugGc -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 56036 | 0.67 | 0.996928 |
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Target: 5'- ---aGUUAGCGguuCGGCGGgcggCG-CCGa -3' miRNA: 3'- aaaaUAAUCGUu--GCCGCCaa--GCuGGC- -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 1365 | 0.67 | 0.995702 |
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Target: 5'- ---gGUUGGgaCGACGGCGGcaauaaUCGGCCu -3' miRNA: 3'- aaaaUAAUC--GUUGCCGCCa-----AGCUGGc -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 50872 | 0.67 | 0.994953 |
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Target: 5'- -----gUGGCGGCGGUGGUugcaUCGcgggcacaggcGCCGg -3' miRNA: 3'- aaaauaAUCGUUGCCGCCA----AGC-----------UGGC- -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 37269 | 0.69 | 0.98797 |
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Target: 5'- aUUUAUUGGCAguuuuuuucACGGCGaaaUGACCGu -3' miRNA: 3'- aAAAUAAUCGU---------UGCCGCcaaGCUGGC- -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 87439 | 0.69 | 0.98797 |
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Target: 5'- -----gUGGCGGCGGCGGcggUGACgGu -3' miRNA: 3'- aaaauaAUCGUUGCCGCCaa-GCUGgC- -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 33474 | 0.7 | 0.969191 |
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Target: 5'- -cUUGUUGguGCGGCGGCGGaUCGGgCa -3' miRNA: 3'- aaAAUAAU--CGUUGCCGCCaAGCUgGc -5' |
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| 7583 | 5' | -48.2 | NC_001962.1 | + | 101723 | 0.71 | 0.958472 |
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Target: 5'- ----uUUGGCGGCGGCGGUUaguuCCa -3' miRNA: 3'- aaaauAAUCGUUGCCGCCAAgcu-GGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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