miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7589 3' -53.5 NC_001963.1 + 2414 0.66 0.152118
Target:  5'- gGGCCGGgGCGu-GGCAucuGAGgGGCg -3'
miRNA:   3'- -CUGGUCaCGUuuCCGUuu-CUCgCCGg -5'
7589 3' -53.5 NC_001963.1 + 2792 0.66 0.162751
Target:  5'- cGACC--UGCAGagggaacggcAGGCAGAGAagagacacaGCGGCa -3'
miRNA:   3'- -CUGGucACGUU----------UCCGUUUCU---------CGCCGg -5'
7589 3' -53.5 NC_001963.1 + 682 0.69 0.093816
Target:  5'- cGACCAGgacGCGAuucGCGGGGGGCGcCCa -3'
miRNA:   3'- -CUGGUCa--CGUUuc-CGUUUCUCGCcGG- -5'
7589 3' -53.5 NC_001963.1 + 1023 1.11 0.000024
Target:  5'- gGACCAGUGCAAAGGCAAAGAGCGGCCc -3'
miRNA:   3'- -CUGGUCACGUUUCCGUUUCUCGCCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.