Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
759 | 3' | -50 | NC_000852.3 | + | 74534 | 0.66 | 0.999928 |
Target: 5'- --uGUACCAGAGGguGCCCauaauuuGGaUGCCa -3' miRNA: 3'- cuuCAUGGUCUCU--UGGGgcu----UC-AUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 186469 | 0.66 | 0.999907 |
Target: 5'- aAAGUAUCGGaAGuucUUCCGAGGUGCUa -3' miRNA: 3'- cUUCAUGGUC-UCuu-GGGGCUUCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 142553 | 0.66 | 0.999907 |
Target: 5'- --uGUACCAcGAGAACCCUc---UGCCu -3' miRNA: 3'- cuuCAUGGU-CUCUUGGGGcuucAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 58455 | 0.67 | 0.999807 |
Target: 5'- cGGAGUGUCGGAGGACUgCGGcGGaACCg -3' miRNA: 3'- -CUUCAUGGUCUCUUGGgGCU-UCaUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 165274 | 0.67 | 0.999756 |
Target: 5'- aGAGGUcACCGGuucGACCCCGGuuGGUGUCg -3' miRNA: 3'- -CUUCA-UGGUCuc-UUGGGGCU--UCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 178560 | 0.67 | 0.99962 |
Target: 5'- -cGGUACCAG-GGACCCU----UACCg -3' miRNA: 3'- cuUCAUGGUCuCUUGGGGcuucAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 147991 | 0.67 | 0.99962 |
Target: 5'- aGAAGUugUAGAGAAgCUgGAAG-AUCu -3' miRNA: 3'- -CUUCAugGUCUCUUgGGgCUUCaUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 156742 | 0.67 | 0.99962 |
Target: 5'- ---aUACCGcGAGAAgCCCGAAGcugaACCu -3' miRNA: 3'- cuucAUGGU-CUCUUgGGGCUUCa---UGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 8776 | 0.67 | 0.999529 |
Target: 5'- --uGUAUCAaAGAACuCCUGAcGGUACCa -3' miRNA: 3'- cuuCAUGGUcUCUUG-GGGCU-UCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 284964 | 0.68 | 0.999421 |
Target: 5'- cGAGG-ACCcGAGGA-CCCGAGGaACCc -3' miRNA: 3'- -CUUCaUGGuCUCUUgGGGCUUCaUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 224388 | 0.68 | 0.998935 |
Target: 5'- --uGUACCAcGGGAAagUCCCGAauucauuuacaauAGUGCCc -3' miRNA: 3'- cuuCAUGGU-CUCUU--GGGGCU-------------UCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 196602 | 0.69 | 0.998202 |
Target: 5'- aGGAGcGCagaAGAcGACUUCGAAGUACCa -3' miRNA: 3'- -CUUCaUGg--UCUcUUGGGGCUUCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 88178 | 0.69 | 0.997479 |
Target: 5'- aGAAGUAuauUCGGAGcauGGCCUCGAAGUucuaACCc -3' miRNA: 3'- -CUUCAU---GGUCUC---UUGGGGCUUCA----UGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 283491 | 0.7 | 0.995953 |
Target: 5'- aGGAGUGCCGGAGAAUaugugacauccuUCCGGAugaucuUGCCa -3' miRNA: 3'- -CUUCAUGGUCUCUUG------------GGGCUUc-----AUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 230793 | 0.7 | 0.995302 |
Target: 5'- ---aUACCGGAGAuGCCUCGuuGUAUCa -3' miRNA: 3'- cuucAUGGUCUCU-UGGGGCuuCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 208619 | 0.7 | 0.993743 |
Target: 5'- aGAAGUACCAGAGAACCCn-------- -3' miRNA: 3'- -CUUCAUGGUCUCUUGGGgcuucaugg -5' |
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759 | 3' | -50 | NC_000852.3 | + | 330028 | 0.71 | 0.989391 |
Target: 5'- ---uUGCCAGAGAACauauacaaCCGAAG-ACCu -3' miRNA: 3'- cuucAUGGUCUCUUGg-------GGCUUCaUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 716 | 0.71 | 0.989391 |
Target: 5'- ---uUGCCAGAGAACauauacaaCCGAAG-ACCu -3' miRNA: 3'- cuucAUGGUCUCUUGg-------GGCUUCaUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 255528 | 0.73 | 0.968388 |
Target: 5'- aGAGUACCAGAcgaugagugGGACCCUGcAAGUAUUc -3' miRNA: 3'- cUUCAUGGUCU---------CUUGGGGC-UUCAUGG- -5' |
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759 | 3' | -50 | NC_000852.3 | + | 208457 | 1.12 | 0.015736 |
Target: 5'- cGAAGUACCAGAGAACCCCGAAGUACCc -3' miRNA: 3'- -CUUCAUGGUCUCUUGGGGCUUCAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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