miRNA: 3'- -CUUCAUGGUCUCUUGGgGCU-UCaUGG- -5'
miRNA: 3'- cuuCAUGGU-CUCUUGGGGcuucAUGG- -5'
miRNA: 3'- cUUCAUGGUC-UCuu-GGGGCUUCAUGG- -5'
miRNA: 3'- cuuCAUGGUCUCU--UGGGgcu----UC-AUGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 759 | 5' | -50 | NC_000852.3 | + | 58455 | 0.67 | 0.999807 |
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Target: 5'- cGGAGUGUCGGAGGACUgCGGcGGaACCg -3' miRNA: 3'- -CUUCAUGGUCUCUUGGgGCU-UCaUGG- -5' |
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| 759 | 5' | -50 | NC_000852.3 | + | 142553 | 0.66 | 0.999907 |
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Target: 5'- --uGUACCAcGAGAACCCUc---UGCCu -3' miRNA: 3'- cuuCAUGGU-CUCUUGGGGcuucAUGG- -5' |
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| 759 | 5' | -50 | NC_000852.3 | + | 186469 | 0.66 | 0.999907 |
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Target: 5'- aAAGUAUCGGaAGuucUUCCGAGGUGCUa -3' miRNA: 3'- cUUCAUGGUC-UCuu-GGGGCUUCAUGG- -5' |
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| 759 | 5' | -50 | NC_000852.3 | + | 74534 | 0.66 | 0.999928 |
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Target: 5'- --uGUACCAGAGGguGCCCauaauuuGGaUGCCa -3' miRNA: 3'- cuuCAUGGUCUCU--UGGGgcu----UC-AUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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