miRNA: 3'- -GGC-GUCCGGaauUCUG-UUCGGUCCGa -5'
miRNA: 3'- -GGCGUCCGGAAUUCUGUUCGGUCCGA- -5'
Results 1 - 2 of 2 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7597 | 3' | -55.1 | NC_001963.1 | + | 2568 | 0.7 | 0.058836 |
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Target: 5'- cCCGuCAGGCU---GGGCgGGGCCGGGUg -3' miRNA: 3'- -GGC-GUCCGGaauUCUG-UUCGGUCCGa -5' |
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| 7597 | 3' | -55.1 | NC_001963.1 | + | 3212 | 1.1 | 0.000019 |
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Target: 5'- gCCGCAGGCCUUAAGACAAGCCAGGCUg -3' miRNA: 3'- -GGCGUCCGGAAUUCUGUUCGGUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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