Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
760 | 3' | -54.7 | NC_000852.3 | + | 217108 | 0.66 | 0.994126 |
Target: 5'- aAGAcuGCaguCCGAGGUAcccCCGGAGAAagagucuccUCCCg -3' miRNA: 3'- -UCU--UGg--GGCUUCAU---GGCCUCUU---------GGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 227242 | 0.66 | 0.993285 |
Target: 5'- uGGcAGCUUCGcGGGUGCUgGGAGAGgCCCg -3' miRNA: 3'- -UC-UUGGGGC-UUCAUGG-CCUCUUgGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 257247 | 0.66 | 0.993285 |
Target: 5'- -uGACCCgGuuGAGUugcACCGGAaacAACCCCa -3' miRNA: 3'- ucUUGGGgC--UUCA---UGGCCUc--UUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 142533 | 0.66 | 0.992152 |
Target: 5'- gAGGACCuuGuagucuggauuGUACCacGAGAACCCUc -3' miRNA: 3'- -UCUUGGggCuu---------CAUGGc-CUCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 230804 | 0.66 | 0.991315 |
Target: 5'- aAGAACa-UGAuaUACCGGAGAugCCUc -3' miRNA: 3'- -UCUUGggGCUucAUGGCCUCUugGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 265529 | 0.68 | 0.980656 |
Target: 5'- uGAACCCCGAuauAGaGCCcGAGAucguuuucuucGCCCa -3' miRNA: 3'- uCUUGGGGCU---UCaUGGcCUCU-----------UGGGg -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 146597 | 0.68 | 0.976305 |
Target: 5'- uGAugUCCGaAAGUAcucCCGGAGAACUa- -3' miRNA: 3'- uCUugGGGC-UUCAU---GGCCUCUUGGgg -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 151241 | 0.7 | 0.939146 |
Target: 5'- -aAGCCCCGAGGguuuugUCGG-GAAUCCCu -3' miRNA: 3'- ucUUGGGGCUUCau----GGCCuCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 151467 | 0.71 | 0.902411 |
Target: 5'- aAGAACCCCGcacuGGggaCGGAGAACUUg -3' miRNA: 3'- -UCUUGGGGCu---UCaugGCCUCUUGGGg -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 284971 | 0.72 | 0.869874 |
Target: 5'- gAGAA-CCCGAGG-ACCcGAGGACCCg -3' miRNA: 3'- -UCUUgGGGCUUCaUGGcCUCUUGGGg -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208626 | 0.75 | 0.72798 |
Target: 5'- -aAACUauaGAAGUACCaGAGAACCCCg -3' miRNA: 3'- ucUUGGgg-CUUCAUGGcCUCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 171724 | 0.76 | 0.660192 |
Target: 5'- -cGGCCCCGAAGUcgcggcgACCGGAGGuuuCCaCCa -3' miRNA: 3'- ucUUGGGGCUUCA-------UGGCCUCUu--GG-GG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208447 | 0.87 | 0.208703 |
Target: 5'- gAGAACCCCGAAGUACCcGA-AACCCCu -3' miRNA: 3'- -UCUUGGGGCUUCAUGGcCUcUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208483 | 1.13 | 0.005644 |
Target: 5'- gAGAACCCCGAAGUACCGGAGAACCCCg -3' miRNA: 3'- -UCUUGGGGCUUCAUGGCCUCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208519 | 1.13 | 0.005644 |
Target: 5'- gAGAACCCCGAAGUACCGGAGAACCCCg -3' miRNA: 3'- -UCUUGGGGCUUCAUGGCCUCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208555 | 1.13 | 0.005644 |
Target: 5'- gAGAACCCCGAAGUACCGGAGAACCCCg -3' miRNA: 3'- -UCUUGGGGCUUCAUGGCCUCUUGGGG- -5' |
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760 | 3' | -54.7 | NC_000852.3 | + | 208591 | 1.13 | 0.005644 |
Target: 5'- gAGAACCCCGAAGUACCGGAGAACCCCg -3' miRNA: 3'- -UCUUGGGGCUUCAUGGCCUCUUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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