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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 7602 | 5' | -54.3 | NC_001963.1 | + | 1113 | 0.66 | 0.173127 |
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Target: 5'- uGGCGGGAUUCauuucgGACUGgAGccuuACGCCg -3' miRNA: 3'- gCUGCCCUAAGg-----CUGGC-UCcu--UGUGG- -5' |
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| 7602 | 5' | -54.3 | NC_001963.1 | + | 3313 | 0.67 | 0.139698 |
|
Target: 5'- aGACGGcGcgUCCGAguaaagucugagcuuCacaGAGGAcaGCACCg -3' miRNA: 3'- gCUGCC-CuaAGGCU---------------Gg--CUCCU--UGUGG- -5' |
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| 7602 | 5' | -54.3 | NC_001963.1 | + | 2423 | 0.69 | 0.102242 |
|
Target: 5'- -uGCGGG-UUCgGGCCGGGGcguGGCAUCu -3' miRNA: 3'- gcUGCCCuAAGgCUGGCUCC---UUGUGG- -5' |
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| 7602 | 5' | -54.3 | NC_001963.1 | + | 4230 | 0.74 | 0.039212 |
|
Target: 5'- uGGCGGGGUUaaugcgacaaCCGACgGAGcGGACACg -3' miRNA: 3'- gCUGCCCUAA----------GGCUGgCUC-CUUGUGg -5' |
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| 7602 | 5' | -54.3 | NC_001963.1 | + | 1982 | 1 | 0.000312 |
|
Target: 5'- aCGACGGGAUUCCGA-CGAGGAACACCu -3' miRNA: 3'- -GCUGCCCUAAGGCUgGCUCCUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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