Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
7611 | 3' | -52.7 | NC_001964.1 | + | 2643 | 0.66 | 0.175126 |
Target: 5'- gUACUUUaccgUCGGCgCCUUCagCGGCAGGgGg -3' miRNA: 3'- -AUGAAA----AGUCG-GGAAG--GCCGUCUgUg -5' |
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7611 | 3' | -52.7 | NC_001964.1 | + | 542 | 0.66 | 0.163635 |
Target: 5'- gACUgUUCGGUgCCgaucaaUCCGGCGGAgGCc -3' miRNA: 3'- aUGAaAAGUCG-GGa-----AGGCCGUCUgUG- -5' |
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7611 | 3' | -52.7 | NC_001964.1 | + | 4910 | 0.88 | 0.002451 |
Target: 5'- gUACUUUUCAaggcaCCCUUCCGGCGGGCGCg -3' miRNA: 3'- -AUGAAAAGUc----GGGAAGGCCGUCUGUG- -5' |
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7611 | 3' | -52.7 | NC_001964.1 | + | 155 | 1.09 | 3.8e-05 |
Target: 5'- aUACUUUUCAGCCCUUCCGGCAGACACg -3' miRNA: 3'- -AUGAAAAGUCGGGAAGGCCGUCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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