Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7612 | 3' | -54.8 | NC_001964.1 | + | 1543 | 0.66 | 0.176428 |
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Target: 5'- aCGgUCGAUGCGGU---GACCGUCCu -3' miRNA: 3'- aGCaAGCUGUGUCGcagCUGGCAGGc -5' |
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| 7612 | 3' | -54.8 | NC_001964.1 | + | 3747 | 0.66 | 0.155076 |
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Target: 5'- gCGUUCGaACACuuacucgcgAGCGUCGAgucCCGUUgGc -3' miRNA: 3'- aGCAAGC-UGUG---------UCGCAGCU---GGCAGgC- -5' |
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| 7612 | 3' | -54.8 | NC_001964.1 | + | 806 | 0.66 | 0.150116 |
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Target: 5'- aCGgUCGACGCuGUGUCGAacgaccaCGUCUu -3' miRNA: 3'- aGCaAGCUGUGuCGCAGCUg------GCAGGc -5' |
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| 7612 | 3' | -54.8 | NC_001964.1 | + | 1826 | 0.69 | 0.0944 |
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Target: 5'- -aGUUCGACuCGGCuUCGGUCGUCCu -3' miRNA: 3'- agCAAGCUGuGUCGcAGCUGGCAGGc -5' |
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| 7612 | 3' | -54.8 | NC_001964.1 | + | 758 | 0.69 | 0.0944 |
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Target: 5'- ---cUUGACucgccccagACGGCGUCGACCGcgUCCGu -3' miRNA: 3'- agcaAGCUG---------UGUCGCAGCUGGC--AGGC- -5' |
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| 7612 | 3' | -54.8 | NC_001964.1 | + | 833 | 1.09 | 0.000046 |
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Target: 5'- gUCGUUCGACACAGCGUCGACCGUCCGc -3' miRNA: 3'- -AGCAAGCUGUGUCGCAGCUGGCAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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