Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7613 | 5' | -51.7 | NC_001964.1 | + | 1198 | 0.66 | 0.235523 |
Target: 5'- gCCacCGACGCGAGCGGcGGA--AGGCc -3' miRNA: 3'- -GGa-GUUGUGUUCGUCaCCUcaUCCGc -5' |
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7613 | 5' | -51.7 | NC_001964.1 | + | 2588 | 0.66 | 0.220806 |
Target: 5'- -gUCAACGgGAgGCGGaacgGGAGgGGGCGg -3' miRNA: 3'- ggAGUUGUgUU-CGUCa---CCUCaUCCGC- -5' |
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7613 | 5' | -51.7 | NC_001964.1 | + | 4947 | 0.68 | 0.171876 |
Target: 5'- aUCUCGGCAaccuccugcgggcgAGGCGGaGGAGUGGGUa -3' miRNA: 3'- -GGAGUUGUg-------------UUCGUCaCCUCAUCCGc -5' |
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7613 | 5' | -51.7 | NC_001964.1 | + | 976 | 0.96 | 0.000859 |
Target: 5'- gCCUCAACACAAGCAGUGGuGaAGGCGg -3' miRNA: 3'- -GGAGUUGUGUUCGUCACCuCaUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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