Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 762 | 3' | -54.5 | NC_000852.3 | + | 30193 | 0.66 | 0.989008 |
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Target: 5'- gCAAGAGcCGCGgUCGUUUCGUUUAGaCUa -3' miRNA: 3'- -GUUCUU-GCGUgGGCAAAGCGGGUC-GA- -5' |
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| 762 | 3' | -54.5 | NC_000852.3 | + | 231464 | 0.66 | 0.987611 |
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Target: 5'- aGAGAACGgGCCCGUaaauaUCGUaUAGCa -3' miRNA: 3'- gUUCUUGCgUGGGCAa----AGCGgGUCGa -5' |
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| 762 | 3' | -54.5 | NC_000852.3 | + | 283205 | 0.68 | 0.968179 |
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Target: 5'- -uGGGACGUugCCGUUUCuUgCAGCg -3' miRNA: 3'- guUCUUGCGugGGCAAAGcGgGUCGa -5' |
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| 762 | 3' | -54.5 | NC_000852.3 | + | 251623 | 0.68 | 0.958439 |
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Target: 5'- --uGAACGUACCCGaugucuucUUUCGCCCuAGaCg -3' miRNA: 3'- guuCUUGCGUGGGC--------AAAGCGGG-UC-Ga -5' |
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| 762 | 3' | -54.5 | NC_000852.3 | + | 188020 | 0.69 | 0.945561 |
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Target: 5'- aCGAGAccugauGCGCauggcauuuuaguuGCCCGUUaCGCCCAGg- -3' miRNA: 3'- -GUUCU------UGCG--------------UGGGCAAaGCGGGUCga -5' |
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| 762 | 3' | -54.5 | NC_000852.3 | + | 210915 | 1.08 | 0.009539 |
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Target: 5'- cCAAGAACGCACCCGUUUCGCCCAGCUa -3' miRNA: 3'- -GUUCUUGCGUGGGCAAAGCGGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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