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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 762 | 5' | -47.9 | NC_000852.3 | + | 135366 | 0.66 | 0.999993 |
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Target: 5'- gGUAaagaUUGGUCGAACAGCgACuGGUUUAc -3' miRNA: 3'- -CAUg---GACUAGCUUGUCGaUG-UCAAGU- -5' |
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| 762 | 5' | -47.9 | NC_000852.3 | + | 103874 | 0.67 | 0.999956 |
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Target: 5'- uUGCggauaUGAUCGuGGCAGCUGCAGguaUCGu -3' miRNA: 3'- cAUGg----ACUAGC-UUGUCGAUGUCa--AGU- -5' |
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| 762 | 5' | -47.9 | NC_000852.3 | + | 128899 | 0.69 | 0.999584 |
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Target: 5'- cUGCCUGAUCGAauuuacgacccAUAGUaacACAGUUCc -3' miRNA: 3'- cAUGGACUAGCU-----------UGUCGa--UGUCAAGu -5' |
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| 762 | 5' | -47.9 | NC_000852.3 | + | 263685 | 0.73 | 0.989441 |
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Target: 5'- -cGCCUGcUCGAGCAGCUcaugcggaugcgACAGUgugUCAc -3' miRNA: 3'- caUGGACuAGCUUGUCGA------------UGUCA---AGU- -5' |
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| 762 | 5' | -47.9 | NC_000852.3 | + | 210880 | 1.1 | 0.031878 |
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Target: 5'- aGUACCUGAUCGAACAGCUACAGUUCAc -3' miRNA: 3'- -CAUGGACUAGCUUGUCGAUGUCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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