Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7622 | 5' | -60.6 | NC_001964.1 | + | 1182 | 0.66 | 0.053552 |
Target: 5'- cGCGUcGGUGGCCCAgagUGCUGuuaGCGccuGGGu -3' miRNA: 3'- uCGCA-CCACCGGGU---GUGGC---UGCu--CCC- -5' |
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7622 | 5' | -60.6 | NC_001964.1 | + | 2405 | 0.67 | 0.050086 |
Target: 5'- uGCGUGGUcGGCCCGaggggcggacCACgGugGgcugguaccugAGGGg -3' miRNA: 3'- uCGCACCA-CCGGGU----------GUGgCugC-----------UCCC- -5' |
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7622 | 5' | -60.6 | NC_001964.1 | + | 1308 | 0.68 | 0.040948 |
Target: 5'- cAGcCGUGGUGacguGCCCGCugCGccagguacagcCGAGGGc -3' miRNA: 3'- -UC-GCACCAC----CGGGUGugGCu----------GCUCCC- -5' |
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7622 | 5' | -60.6 | NC_001964.1 | + | 1059 | 1.09 | 8e-06 |
Target: 5'- gAGCGUGGUGGCCCACACCGACGAGGGc -3' miRNA: 3'- -UCGCACCACCGGGUGUGGCUGCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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