Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7626 | 5' | -60 | NC_001964.1 | + | 4936 | 0.68 | 0.047213 |
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Target: 5'- gCCGAgaucuguacuacAGCGGG-CUAUCGCCgAGGCaGGu -3' miRNA: 3'- -GGCU------------UCGCCUaGGUGGCGG-UCCGcCC- -5' |
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| 7626 | 5' | -60 | NC_001964.1 | + | 374 | 0.68 | 0.045661 |
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Target: 5'- cCCGccuuGCGGGUCgGCUagaguGCCcacuaaagGGGCGGGg -3' miRNA: 3'- -GGCuu--CGCCUAGgUGG-----CGG--------UCCGCCC- -5' |
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| 7626 | 5' | -60 | NC_001964.1 | + | 4721 | 0.7 | 0.031565 |
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Target: 5'- aCUGAAGCGGGgauuggCCuuACUGCCGugauGGCGGa -3' miRNA: 3'- -GGCUUCGCCUa-----GG--UGGCGGU----CCGCCc -5' |
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| 7626 | 5' | -60 | NC_001964.1 | + | 2957 | 0.71 | 0.025782 |
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Target: 5'- cCUGGcGGUGGAUCCGCUucgGaaAGGCGGGg -3' miRNA: 3'- -GGCU-UCGCCUAGGUGG---CggUCCGCCC- -5' |
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| 7626 | 5' | -60 | NC_001964.1 | + | 2931 | 1.12 | 6e-06 |
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Target: 5'- uCCGAAGCGGAUCCACCGCCAGGCGGGg -3' miRNA: 3'- -GGCUUCGCCUAGGUGGCGGUCCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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