Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
763 | 5' | -50.7 | NC_000852.3 | + | 122982 | 0.66 | 0.999715 |
Target: 5'- gCGCgGGCuuCGGUGCAGGcuUCGgCUc -3' miRNA: 3'- -GCG-CCGuuGCUACGUCUuuAGCaGAa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 255153 | 0.66 | 0.999715 |
Target: 5'- cCGCGGUAA-GAauacuUGCAGGGucccacucAUCGUCUg -3' miRNA: 3'- -GCGCCGUUgCU-----ACGUCUU--------UAGCAGAa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 246511 | 0.66 | 0.999643 |
Target: 5'- cCGCGGUAugGAUucucuaaagcaGCAGGcaGGUCGUa-- -3' miRNA: 3'- -GCGCCGUugCUA-----------CGUCU--UUAGCAgaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 26759 | 0.66 | 0.999557 |
Target: 5'- uCGUuuucaGACgGAUGCAGAAAUCGUCa- -3' miRNA: 3'- -GCGccg--UUG-CUACGUCUUUAGCAGaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 120324 | 0.66 | 0.999557 |
Target: 5'- aGCGGCAuuuuuUGCAGAAcuugacaauUCGUCUa -3' miRNA: 3'- gCGCCGUugcu-ACGUCUUu--------AGCAGAa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 175022 | 0.66 | 0.999453 |
Target: 5'- uCGuCGGUuucacCGGUGUAGAuAUCGUCa- -3' miRNA: 3'- -GC-GCCGuu---GCUACGUCUuUAGCAGaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 308400 | 0.67 | 0.998797 |
Target: 5'- uCGCGGUuccGCGuagaGCAGGuGAUCGUCUc -3' miRNA: 3'- -GCGCCGu--UGCua--CGUCU-UUAGCAGAa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 103862 | 0.67 | 0.998554 |
Target: 5'- uCGUGGCAGC--UGCAGguAUCGUa-- -3' miRNA: 3'- -GCGCCGUUGcuACGUCuuUAGCAgaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 81761 | 0.68 | 0.997563 |
Target: 5'- cCGCGGUugacuuUGGUGCAGggG-CGUCc- -3' miRNA: 3'- -GCGCCGuu----GCUACGUCuuUaGCAGaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 264400 | 0.69 | 0.993876 |
Target: 5'- --aGGCGAUGAUGUGGAugcuaaagcgagAGUCGUCUc -3' miRNA: 3'- gcgCCGUUGCUACGUCU------------UUAGCAGAa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 45926 | 0.7 | 0.990804 |
Target: 5'- cCGCGGaugcuguCGuugGCGGAAAUCGUCa- -3' miRNA: 3'- -GCGCCguu----GCua-CGUCUUUAGCAGaa -5' |
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763 | 5' | -50.7 | NC_000852.3 | + | 260550 | 1.06 | 0.028994 |
Target: 5'- cCGCGGCAACGAUGCAGAAAUCGUCUUu -3' miRNA: 3'- -GCGCCGUUGCUACGUCUUUAGCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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