miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7632 3' -60.1 NC_001964.1 + 4979 0.97 0.000092
Target:  5'- aAUCCGGACGCC-CCGACCUCGAUCUCg -3'
miRNA:   3'- -UAGGCCUGCGGcGGCUGGAGCUAGAG- -5'
7632 3' -60.1 NC_001964.1 + 2827 0.71 0.022285
Target:  5'- -gCCGGugGgCCGCCGGCCUUGccaCUg -3'
miRNA:   3'- uaGGCCugC-GGCGGCUGGAGCua-GAg -5'
7632 3' -60.1 NC_001964.1 + 1377 0.66 0.059939
Target:  5'- gGUCgCGcGGCagaugGCCGCCGACCUaGGUCg- -3'
miRNA:   3'- -UAG-GC-CUG-----CGGCGGCUGGAgCUAGag -5'
7632 3' -60.1 NC_001964.1 + 4062 0.66 0.061994
Target:  5'- uGUCUGGACGCCGCgggaCGACUUacAUCa- -3'
miRNA:   3'- -UAGGCCUGCGGCG----GCUGGAgcUAGag -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.