Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7633 | 5' | -55.1 | NC_001973.1 | + | 160497 | 0.74 | 0.583309 |
Target: 5'- cGGCCaGGGUCGA---UgGAGGUCGAGc -3' miRNA: 3'- -CCGGcCUCAGCUaguAgCUCCAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 84128 | 0.74 | 0.583309 |
Target: 5'- cGGCCGaucucagaaaucGAGUCGcgCAacUCGAGGcCGAGg -3' miRNA: 3'- -CCGGC------------CUCAGCuaGU--AGCUCCaGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 124808 | 0.77 | 0.449214 |
Target: 5'- gGGCCGGGGccUCGAUCGgac-GGUCGAGg -3' miRNA: 3'- -CCGGCCUC--AGCUAGUagcuCCAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 109298 | 0.77 | 0.422661 |
Target: 5'- --aCGGAG-CGcgCGUCGGGGUCGAGg -3' miRNA: 3'- ccgGCCUCaGCuaGUAGCUCCAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 130808 | 0.8 | 0.319312 |
Target: 5'- cGGCCGGGGUCGAUCGaguUCGAGccCGAu -3' miRNA: 3'- -CCGGCCUCAGCUAGU---AGCUCcaGCUc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 160831 | 1.1 | 0.003735 |
Target: 5'- cGGCCGGAGUCGAUCAUCGAGGUCGAGc -3' miRNA: 3'- -CCGGCCUCAGCUAGUAGCUCCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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