Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7635 | 5' | -58.5 | NC_001973.1 | + | 14351 | 0.66 | 0.836843 |
Target: 5'- cUugGCGUGGUCgGcGUgGUCGA-CGGGg -3' miRNA: 3'- -AugCGCACCGGgC-CA-CAGCUaGCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 12137 | 0.66 | 0.828697 |
Target: 5'- uUACGCGUcuGCCgGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCAc-CGGgCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 130722 | 0.66 | 0.820379 |
Target: 5'- uUACGCGUcGGCCgGGg--CaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGGgCCacaGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 72051 | 0.66 | 0.811898 |
Target: 5'- uUACGCGUcGGCgaGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 71964 | 0.66 | 0.811898 |
Target: 5'- uUACGCGUcGGCgaGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 72220 | 0.66 | 0.806734 |
Target: 5'- gACGCGUaagauuuuacgugccGGCCgGG-GUCaAUCGAGUu -3' miRNA: 3'- aUGCGCA---------------CCGGgCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 16450 | 0.66 | 0.806734 |
Target: 5'- gACGCGUaagauuuuacgugccGGCCgGG-GUCaAUCGAGUu -3' miRNA: 3'- aUGCGCA---------------CCGGgCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 151376 | 0.67 | 0.758008 |
Target: 5'- uUACGCGUcGGCCgGG-GUCGucUGAGg -3' miRNA: 3'- -AUGCGCA-CCGGgCCaCAGCuaGCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 12372 | 0.67 | 0.748603 |
Target: 5'- uUACGCGUcGGCaaGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 20987 | 0.67 | 0.748603 |
Target: 5'- uUACGCGUcGGCuaGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 132398 | 0.67 | 0.748603 |
Target: 5'- gACGCGUcagauccuccucGGCCCGGa-UCGAgagCGAGg -3' miRNA: 3'- aUGCGCA------------CCGGGCCacAGCUa--GCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 59878 | 0.68 | 0.71009 |
Target: 5'- cGCGCGcGGCCCcaacggcggcgcGGUGUCGGccaacgCGAGc -3' miRNA: 3'- aUGCGCaCCGGG------------CCACAGCUa-----GCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 72146 | 0.69 | 0.650583 |
Target: 5'- uUACGCGUcGGCaaag-GUCGAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggccaCAGCUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 160823 | 0.69 | 0.640569 |
Target: 5'- uUACGCGUcGG-CCGGaGUCGAucaUCGAGg -3' miRNA: 3'- -AUGCGCA-CCgGGCCaCAGCU---AGCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 95836 | 0.71 | 0.512321 |
Target: 5'- gGCGCGgcgacgGGCCCGGUGcCGGgcacgUGGGa -3' miRNA: 3'- aUGCGCa-----CCGGGCCACaGCUa----GCUCa -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 12202 | 0.73 | 0.442233 |
Target: 5'- gACGCGUaggauuuuacgugccGGCCaGG-GUCGAUCGAGUu -3' miRNA: 3'- aUGCGCA---------------CCGGgCCaCAGCUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 21086 | 0.73 | 0.42124 |
Target: 5'- uUACGCGUcGGCCgGG-GUCaAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGGgCCaCAGcUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 160567 | 0.77 | 0.241776 |
Target: 5'- uUACGCGUcGGCuaGG-GUCGAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGCUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 130898 | 0.77 | 0.241776 |
Target: 5'- uUACGCGUcGGCuaGG-GUCGAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGCUAGCUCA- -5' |
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7635 | 5' | -58.5 | NC_001973.1 | + | 16541 | 0.77 | 0.241776 |
Target: 5'- uUACGCGUcGGCuaGG-GUCGAUCGAGUu -3' miRNA: 3'- -AUGCGCA-CCGggCCaCAGCUAGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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