Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7637 | 5' | -53.9 | NC_001973.1 | + | 106611 | 0.66 | 0.962582 |
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Target: 5'- cGAGCGCGAccGG--CGUCAAGAGAc -3' miRNA: 3'- cCUCGCGUUcuCCuaGCGGUUCUCUa -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 42611 | 0.66 | 0.962582 |
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Target: 5'- -cGGCGCGAGGGcgcGGUCGCCAcGGAa-- -3' miRNA: 3'- ccUCGCGUUCUC---CUAGCGGU-UCUcua -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 24933 | 0.66 | 0.962582 |
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Target: 5'- --cGCGCAacgcgccguacAGAGGAUCGgCAAGGcGGUc -3' miRNA: 3'- ccuCGCGU-----------UCUCCUAGCgGUUCU-CUA- -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 153635 | 0.66 | 0.958978 |
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Target: 5'- aGGAGCGCGcGGAGGcaAUCaaaauGUUGAGAGAc -3' miRNA: 3'- -CCUCGCGU-UCUCC--UAG-----CGGUUCUCUa -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 5704 | 0.66 | 0.958978 |
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Target: 5'- aGGAGUGCcuGAGGAgcgcgUCGCCccgcuGGGuGGUg -3' miRNA: 3'- -CCUCGCGuuCUCCU-----AGCGG-----UUCuCUA- -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 142600 | 0.69 | 0.860694 |
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Target: 5'- cGGGGCGCAAGcGGAggCGCUccgAAGGGc- -3' miRNA: 3'- -CCUCGCGUUCuCCUa-GCGG---UUCUCua -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 7463 | 0.7 | 0.801281 |
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Target: 5'- -cGGCGCGAcGAGGAcuuUUGCCGcAGAGAa -3' miRNA: 3'- ccUCGCGUU-CUCCU---AGCGGU-UCUCUa -5' |
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| 7637 | 5' | -53.9 | NC_001973.1 | + | 159139 | 1.08 | 0.005066 |
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Target: 5'- cGGAGCGCAAGAGGAUCGCCAAGAGAUu -3' miRNA: 3'- -CCUCGCGUUCUCCUAGCGGUUCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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