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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 764 | 5' | -45.5 | NC_000852.3 | + | 262524 | 0.67 | 1 |
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Target: 5'- uCUCAGacgCCAgagAACUUGCGggUUUGGGu -3' miRNA: 3'- -GAGUCaa-GGU---UUGAGCGCuaAGAUUC- -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 195187 | 0.68 | 0.999998 |
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Target: 5'- aCUCAGaauggaUUCCAGGCagauaUGCGAUUCUAc- -3' miRNA: 3'- -GAGUC------AAGGUUUGa----GCGCUAAGAUuc -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 123711 | 0.69 | 0.999996 |
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Target: 5'- -----aUCCAAAgUUCGCGAUUCUAAa -3' miRNA: 3'- gagucaAGGUUU-GAGCGCUAAGAUUc -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 270603 | 0.8 | 0.934968 |
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Target: 5'- -cCAaUUCCAAACcCGCGAUUCUAAGg -3' miRNA: 3'- gaGUcAAGGUUUGaGCGCUAAGAUUC- -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 273815 | 0.97 | 0.268678 |
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Target: 5'- cCUCAGUUCCAGACcCGUGAUUCUAAGg -3' miRNA: 3'- -GAGUCAAGGUUUGaGCGCUAAGAUUC- -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 270746 | 1.12 | 0.045252 |
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Target: 5'- cCUCAGUUCCAAACUCGCGAUUCUAAGg -3' miRNA: 3'- -GAGUCAAGGUUUGAGCGCUAAGAUUC- -5' |
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| 764 | 5' | -45.5 | NC_000852.3 | + | 273674 | 1.12 | 0.044008 |
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Target: 5'- cCUCAGUUCCAGACUCGCGAUUCUAAGg -3' miRNA: 3'- -GAGUCAAGGUUUGAGCGCUAAGAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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