Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7643 | 3' | -53.5 | NC_001973.1 | + | 59535 | 0.66 | 0.977912 |
Target: 5'- -aGACGAugC--GCUUGGACGcGG-CCa -3' miRNA: 3'- gaCUGUUugGauCGAGCCUGC-CCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 64104 | 0.66 | 0.977912 |
Target: 5'- --aACAGGCCgacGGCUCG-ACGGGgcggugCCg -3' miRNA: 3'- gacUGUUUGGa--UCGAGCcUGCCCa-----GG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 140973 | 0.66 | 0.972823 |
Target: 5'- -cGACAucACCUu-CUCGGACGaGG-CCg -3' miRNA: 3'- gaCUGUu-UGGAucGAGCCUGC-CCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 21530 | 0.66 | 0.969982 |
Target: 5'- -cGACGuGCCUgaucucGGCgUCGGGCGcgccguGGUCCc -3' miRNA: 3'- gaCUGUuUGGA------UCG-AGCCUGC------CCAGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 153342 | 0.66 | 0.968179 |
Target: 5'- cCUGAaaaucGCCaagAGCgaggucgauuucgacUCGGACGGGUCg -3' miRNA: 3'- -GACUguu--UGGa--UCG---------------AGCCUGCCCAGg -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 131777 | 0.67 | 0.963676 |
Target: 5'- uUGACGAcggcgGCC-GGCUCGGugaaGGGgCCg -3' miRNA: 3'- gACUGUU-----UGGaUCGAGCCug--CCCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 145127 | 0.67 | 0.960199 |
Target: 5'- uUGAC-GGCCgagcaGGCUCuGGACucGGUCCg -3' miRNA: 3'- gACUGuUUGGa----UCGAG-CCUGc-CCAGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 127495 | 0.67 | 0.948412 |
Target: 5'- aUGACAucauGCCUaaacugAGCUCGG-CGGG-Cg -3' miRNA: 3'- gACUGUu---UGGA------UCGAGCCuGCCCaGg -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 58476 | 0.68 | 0.934516 |
Target: 5'- gCUGcCAAACgU-GCUCGG-CGGG-CCg -3' miRNA: 3'- -GACuGUUUGgAuCGAGCCuGCCCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 150864 | 0.68 | 0.929404 |
Target: 5'- uCUGucCGGGCCUGGC-CGGAgcCGGGgcgcguUCCa -3' miRNA: 3'- -GACu-GUUUGGAUCGaGCCU--GCCC------AGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 61182 | 0.69 | 0.911425 |
Target: 5'- -cGACGcuCCUcgcugaacgcgcGCUCGGGCGGGcgCCg -3' miRNA: 3'- gaCUGUuuGGAu-----------CGAGCCUGCCCa-GG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 38133 | 0.69 | 0.906546 |
Target: 5'- uUGcACGAACgcGGCUUGGAUGGGgcccgCCg -3' miRNA: 3'- gAC-UGUUUGgaUCGAGCCUGCCCa----GG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 68993 | 0.69 | 0.906546 |
Target: 5'- -cGGCGAACCcgucgucuGgaCGGACGGG-CCg -3' miRNA: 3'- gaCUGUUUGGau------CgaGCCUGCCCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 151199 | 0.69 | 0.906546 |
Target: 5'- aUGACAucauGCCUcGGCcggCGGugcCGGGUCUg -3' miRNA: 3'- gACUGUu---UGGA-UCGa--GCCu--GCCCAGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 146114 | 0.69 | 0.900234 |
Target: 5'- -cGGCGcugAGCC-GGCUCGGgaGCGGG-CCg -3' miRNA: 3'- gaCUGU---UUGGaUCGAGCC--UGCCCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 68628 | 0.7 | 0.879905 |
Target: 5'- gCUGACGcaaugGGCCagAGaCUCGGACGcGGcCCu -3' miRNA: 3'- -GACUGU-----UUGGa-UC-GAGCCUGC-CCaGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 55895 | 0.7 | 0.865232 |
Target: 5'- -cGGCAGGCUcgGGCUCGGGCucGGGcUCg -3' miRNA: 3'- gaCUGUUUGGa-UCGAGCCUG--CCC-AGg -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 22772 | 0.71 | 0.841649 |
Target: 5'- -cGACAucGACCUGG-UCGGGCGGcaGUUCa -3' miRNA: 3'- gaCUGU--UUGGAUCgAGCCUGCC--CAGG- -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 56524 | 0.71 | 0.807552 |
Target: 5'- -cGGC--GCCaAGCUCGGGCGGGg-- -3' miRNA: 3'- gaCUGuuUGGaUCGAGCCUGCCCagg -5' |
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7643 | 3' | -53.5 | NC_001973.1 | + | 56497 | 0.71 | 0.807552 |
Target: 5'- -cGGC--GCCaAGCUCGGGCGGGg-- -3' miRNA: 3'- gaCUGuuUGGaUCGAGCCUGCCCagg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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