Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7643 | 5' | -63.9 | NC_001973.1 | + | 85324 | 0.66 | 0.616579 |
Target: 5'- gCGGCUCaCGUUCGAgGUCGGcucuucgagcccGACGCGc -3' miRNA: 3'- aGCCGGGgGCAGGCU-CAGCC------------CUGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 69647 | 0.66 | 0.615628 |
Target: 5'- gCGcGCCCuCCGUUuugcuccgcagcaCGAgGUCGGG-CGCGg -3' miRNA: 3'- aGC-CGGG-GGCAG-------------GCU-CAGCCCuGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 34339 | 0.66 | 0.607082 |
Target: 5'- gUUGaGCUCCCaG-CgCGAGUCGGG-CGCGu -3' miRNA: 3'- -AGC-CGGGGG-CaG-GCUCAGCCCuGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 60980 | 0.66 | 0.607082 |
Target: 5'- aCGucGUCgUCGUUCGAGUCGGGGC-CGa -3' miRNA: 3'- aGC--CGGgGGCAGGCUCAGCCCUGcGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 26545 | 0.67 | 0.578714 |
Target: 5'- gUCGGCCgagcgcaaCCauUCCGAGUCGcGGGCGa- -3' miRNA: 3'- -AGCCGG--------GGgcAGGCUCAGC-CCUGCgc -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 73694 | 0.67 | 0.541373 |
Target: 5'- -gGGgCCCCG-CCGcgcAGUCGacGGGCGCGc -3' miRNA: 3'- agCCgGGGGCaGGC---UCAGC--CCUGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 158437 | 0.67 | 0.532157 |
Target: 5'- aUCGcGCCCgCGUCCGAGcCGGcuCGUc -3' miRNA: 3'- -AGC-CGGGgGCAGGCUCaGCCcuGCGc -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 77295 | 0.67 | 0.532157 |
Target: 5'- cUCcGCCgCCG-CCGAGaCGGGcgGCGCGg -3' miRNA: 3'- -AGcCGGgGGCaGGCUCaGCCC--UGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 26979 | 0.68 | 0.522999 |
Target: 5'- uUCGGUCaCCagacucUCCGuGUCGGG-CGCGg -3' miRNA: 3'- -AGCCGG-GGgc----AGGCuCAGCCCuGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 109216 | 0.68 | 0.522999 |
Target: 5'- cUCGGCCUCgaucgCGUCCaGGUCcGGGAUcaGCGa -3' miRNA: 3'- -AGCCGGGG-----GCAGGcUCAG-CCCUG--CGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 150869 | 0.68 | 0.478203 |
Target: 5'- cCGGgCCUgGcCgGAGcCGGGGCGCGu -3' miRNA: 3'- aGCCgGGGgCaGgCUCaGCCCUGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 69975 | 0.68 | 0.477326 |
Target: 5'- aUGGCCCacuugagaaCGUCCGAGucgucgaUCGGGcCGCc -3' miRNA: 3'- aGCCGGGg--------GCAGGCUC-------AGCCCuGCGc -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 60000 | 0.69 | 0.469469 |
Target: 5'- cCGGCgacCCCCGUCUGAugGUCGGcuacaagaaGACGUGc -3' miRNA: 3'- aGCCG---GGGGCAGGCU--CAGCC---------CUGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 70547 | 0.69 | 0.443769 |
Target: 5'- aCGGCCuCCCGU--GAGcCGGGACcGCa -3' miRNA: 3'- aGCCGG-GGGCAggCUCaGCCCUG-CGc -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 109031 | 0.69 | 0.418065 |
Target: 5'- gUCGGCucguccaCCUCGUCuCGGuuGUCGGGcCGCGg -3' miRNA: 3'- -AGCCG-------GGGGCAG-GCU--CAGCCCuGCGC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 159117 | 0.7 | 0.379345 |
Target: 5'- cUCGGCCCgcgaCUGagCGAGUCGGaGCGCa -3' miRNA: 3'- -AGCCGGG----GGCagGCUCAGCCcUGCGc -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 155952 | 0.72 | 0.295142 |
Target: 5'- gUCGGCCUCCG-CCGAgGUgGuGGACGaCGa -3' miRNA: 3'- -AGCCGGGGGCaGGCU-CAgC-CCUGC-GC- -5' |
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7643 | 5' | -63.9 | NC_001973.1 | + | 154992 | 1.08 | 0.000868 |
Target: 5'- uUCGGCCCCCGUCCGAGUCGGGACGCGa -3' miRNA: 3'- -AGCCGGGGGCAGGCUCAGCCCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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