miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7644 5' -46.9 NC_001973.1 + 46605 0.66 0.999935
Target:  5'- -aAAGCCgcgCGAGGCGccGAAUUCAAG-CAg -3'
miRNA:   3'- gaUUUGG---GCUCCGU--UUUAGGUUCaGU- -5'
7644 5' -46.9 NC_001973.1 + 124695 0.66 0.999913
Target:  5'- ----cCCCGAGGCGGuggCCGAGc-- -3'
miRNA:   3'- gauuuGGGCUCCGUUuuaGGUUCagu -5'
7644 5' -46.9 NC_001973.1 + 115034 0.66 0.999913
Target:  5'- uUGAACCCGAucgaccccGGCGuccgCCGAGcUCGa -3'
miRNA:   3'- gAUUUGGGCU--------CCGUuuuaGGUUC-AGU- -5'
7644 5' -46.9 NC_001973.1 + 26549 0.66 0.999851
Target:  5'- -----gCCGAGcGCAAccauUCCGAGUCGc -3'
miRNA:   3'- gauuugGGCUC-CGUUuu--AGGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 39396 0.67 0.9996
Target:  5'- ---uGCUCGAcGGCGcGcgCCAGGUCGg -3'
miRNA:   3'- gauuUGGGCU-CCGUuUuaGGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 144218 0.67 0.9996
Target:  5'- --cGGCgCCGAGGCcGAAUCgCuGGUCGa -3'
miRNA:   3'- gauUUG-GGCUCCGuUUUAG-GuUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 21507 0.68 0.999372
Target:  5'- --cGACUCGAGGUugc--CCAGGUCGc -3'
miRNA:   3'- gauUUGGGCUCCGuuuuaGGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 26618 0.68 0.999221
Target:  5'- ----uCCCGuuuGGCGGcuUCCAAGUCc -3'
miRNA:   3'- gauuuGGGCu--CCGUUuuAGGUUCAGu -5'
7644 5' -46.9 NC_001973.1 + 78237 0.68 0.999039
Target:  5'- uCUGAACgCGcucaucaaaaaGGGCAAAAUUCGcAGUCAc -3'
miRNA:   3'- -GAUUUGgGC-----------UCCGUUUUAGGU-UCAGU- -5'
7644 5' -46.9 NC_001973.1 + 87199 0.68 0.998823
Target:  5'- aCUGAGCCU-AGGCGAgcacggcggugGAggCCAAGUCGu -3'
miRNA:   3'- -GAUUUGGGcUCCGUU-----------UUa-GGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 133423 0.7 0.994266
Target:  5'- -gAAACUCGAGGaaggagcgcuCAAAGUCgAAGUCGg -3'
miRNA:   3'- gaUUUGGGCUCC----------GUUUUAGgUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 51458 0.72 0.982905
Target:  5'- -cGAGCCCG-GGCGAgcucGAgcggCCGGGUCGa -3'
miRNA:   3'- gaUUUGGGCuCCGUU----UUa---GGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 41826 0.73 0.970013
Target:  5'- -cGAGCCCGAcaccgagccgcGGCugcucgagcugAAGAUCCGAGUCGc -3'
miRNA:   3'- gaUUUGGGCU-----------CCG-----------UUUUAGGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 149054 0.73 0.966769
Target:  5'- ----cCCCGAgGGCAuugccAAGUCCAAGUCc -3'
miRNA:   3'- gauuuGGGCU-CCGU-----UUUAGGUUCAGu -5'
7644 5' -46.9 NC_001973.1 + 109408 0.75 0.931696
Target:  5'- cCUAGGCgCCGGcGCGucGUCCAAGUCGa -3'
miRNA:   3'- -GAUUUG-GGCUcCGUuuUAGGUUCAGU- -5'
7644 5' -46.9 NC_001973.1 + 154251 1.11 0.018029
Target:  5'- cCUAAACCCGAGGCAAAAUCCAAGUCAa -3'
miRNA:   3'- -GAUUUGGGCUCCGUUUUAGGUUCAGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.