Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7646 | 3' | -61.2 | NC_001973.1 | + | 37093 | 0.66 | 0.745582 |
Target: 5'- gAGGACCGcCGCCgAGCCGacUCUGcuUCg -3' miRNA: 3'- -UCCUGGCuGCGGgUUGGCccAGGC--AG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 76719 | 0.66 | 0.745582 |
Target: 5'- cAGGccACCGugcCGUCCAACauacagaGGG-CCGUCg -3' miRNA: 3'- -UCC--UGGCu--GCGGGUUGg------CCCaGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 36323 | 0.66 | 0.745582 |
Target: 5'- -cGGCCGACGCggCCGACgCGGG-CC-UCg -3' miRNA: 3'- ucCUGGCUGCG--GGUUG-GCCCaGGcAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 111072 | 0.66 | 0.717468 |
Target: 5'- --aACgCGGCGCCCGACgUGGGcCgCGUCg -3' miRNA: 3'- uccUG-GCUGCGGGUUG-GCCCaG-GCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 18136 | 0.66 | 0.717468 |
Target: 5'- cGGcGCCGAUGCCCAcGCCcGuUCCGUUc -3' miRNA: 3'- uCC-UGGCUGCGGGU-UGGcCcAGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 84289 | 0.66 | 0.707953 |
Target: 5'- gAGGACCucgaacGGCGCaCGGCCGcGUCCGcCg -3' miRNA: 3'- -UCCUGG------CUGCGgGUUGGCcCAGGCaG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 146063 | 0.66 | 0.69838 |
Target: 5'- uGGGCCGcgGCGCCgAGCCGGcUCgGg- -3' miRNA: 3'- uCCUGGC--UGCGGgUUGGCCcAGgCag -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 128139 | 0.66 | 0.69838 |
Target: 5'- cAGG-CUGugGCaCAGCCgcGGGUCCGa- -3' miRNA: 3'- -UCCuGGCugCGgGUUGG--CCCAGGCag -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 20101 | 0.66 | 0.69838 |
Target: 5'- cAGG-CUGugGCaCAGCCgcGGGUCCGa- -3' miRNA: 3'- -UCCuGGCugCGgGUUGG--CCCAGGCag -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 151369 | 0.66 | 0.688755 |
Target: 5'- uAGGAUCuuACGCgUCGGCCGGGgUCGUCu -3' miRNA: 3'- -UCCUGGc-UGCG-GGUUGGCCCaGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 7267 | 0.66 | 0.68779 |
Target: 5'- cGGGCuCGgcgcgcggcGCGCCCGcuACCGGGUCaagcacaccgcgcUGUCg -3' miRNA: 3'- uCCUG-GC---------UGCGGGU--UGGCCCAG-------------GCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 121378 | 0.66 | 0.686825 |
Target: 5'- uGGGCCGacGCGCCCGucACCGGGaacagcacgcgaUCGUUg -3' miRNA: 3'- uCCUGGC--UGCGGGU--UGGCCCa-----------GGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 151084 | 0.67 | 0.669386 |
Target: 5'- uAGGAUCcuacGCGCCC-GCCGGGgUCGUUu -3' miRNA: 3'- -UCCUGGc---UGCGGGuUGGCCCaGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 124806 | 0.67 | 0.649906 |
Target: 5'- gAGGGCCGGgGCCuCGAUCGGa-CgGUCg -3' miRNA: 3'- -UCCUGGCUgCGG-GUUGGCCcaGgCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 122622 | 0.67 | 0.640144 |
Target: 5'- cGGcGCCGACGCgggcGCCGGcGUCgGUCg -3' miRNA: 3'- uCC-UGGCUGCGggu-UGGCC-CAGgCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 4888 | 0.68 | 0.610849 |
Target: 5'- aAGGGCa-GCGCgUAcGCCGGGUUCGUUa -3' miRNA: 3'- -UCCUGgcUGCGgGU-UGGCCCAGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 21272 | 0.68 | 0.59138 |
Target: 5'- cGaGCCGcgGCGUCC-GCCGGG-CCGUCg -3' miRNA: 3'- uCcUGGC--UGCGGGuUGGCCCaGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 103960 | 0.69 | 0.552814 |
Target: 5'- cGGcGCCaGCaGCCUAGCCGGGgcgugCCGUUg -3' miRNA: 3'- uCC-UGGcUG-CGGGUUGGCCCa----GGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 104011 | 0.72 | 0.360783 |
Target: 5'- cGGGCCGucgacucgcgGCGCCagcaaccUAGCCGGG-CCGUCg -3' miRNA: 3'- uCCUGGC----------UGCGG-------GUUGGCCCaGGCAG- -5' |
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7646 | 3' | -61.2 | NC_001973.1 | + | 153426 | 1.09 | 0.00114 |
Target: 5'- cAGGACCGACGCCCAACCGGGUCCGUCg -3' miRNA: 3'- -UCCUGGCUGCGGGUUGGCCCAGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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