Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7649 | 3' | -52.8 | NC_001973.1 | + | 130600 | 0.72 | 0.719272 |
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Target: 5'- gAUGACAUCAUGCCuaaaccgagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 12250 | 0.72 | 0.719272 |
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Target: 5'- gAUGACAUCAUGCCuaaacccagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 127415 | 0.73 | 0.698425 |
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Target: 5'- gAUGACAUCAUGCCuaaacucgagcUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 129207 | 0.73 | 0.698425 |
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Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 130933 | 0.73 | 0.698425 |
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Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 160602 | 0.73 | 0.698425 |
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Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 133259 | 0.74 | 0.624076 |
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Target: 5'- gAUGGCGUCcagcucgGCCUCGGCCGcggccGAGAAc -3' miRNA: 3'- -UACUGUAGua-----CGGAGCCGGCu----UUCUU- -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 151198 | 0.9 | 0.079678 |
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Target: 5'- gAUGACAUCAUGCCUCGGCCGgcGGu- -3' miRNA: 3'- -UACUGUAGUACGGAGCCGGCuuUCuu -5' |
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| 7649 | 3' | -52.8 | NC_001973.1 | + | 151148 | 1.04 | 0.009375 |
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Target: 5'- gAUGACAUCAUGCCUCGGCCGAAAGAAu -3' miRNA: 3'- -UACUGUAGUACGGAGCCGGCUUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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