Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
765 | 3' | -56 | NC_000852.3 | + | 92122 | 0.66 | 0.974746 |
Target: 5'- gUAGACGCuugugcuuucccAACUCCAGAU-C-UCCCCGc -3' miRNA: 3'- -GUCUGCG------------UUGGGGUCUAuGcAGGGGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 298989 | 0.67 | 0.966557 |
Target: 5'- gAGuCGU--CCCCGGAuuaUACGUCCaCCAg -3' miRNA: 3'- gUCuGCGuuGGGGUCU---AUGCAGG-GGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 99758 | 0.67 | 0.963452 |
Target: 5'- cCAGGCGaucCCCCGGGUGaacCCCCGa -3' miRNA: 3'- -GUCUGCguuGGGGUCUAUgcaGGGGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 116199 | 0.67 | 0.963452 |
Target: 5'- aCAGACGCAuACUCCAGGUAgGUUgUg- -3' miRNA: 3'- -GUCUGCGU-UGGGGUCUAUgCAGgGgu -5' |
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765 | 3' | -56 | NC_000852.3 | + | 116644 | 0.69 | 0.915678 |
Target: 5'- -cGGCGcCAACCCCGGGUGgGUUUCUc -3' miRNA: 3'- guCUGC-GUUGGGGUCUAUgCAGGGGu -5' |
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765 | 3' | -56 | NC_000852.3 | + | 7602 | 0.7 | 0.891891 |
Target: 5'- -uGACGCGucaAUUCCAGggAUGUCCCCc -3' miRNA: 3'- guCUGCGU---UGGGGUCuaUGCAGGGGu -5' |
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765 | 3' | -56 | NC_000852.3 | + | 264709 | 0.73 | 0.756992 |
Target: 5'- aCAGAuCGUuACCCCAGAUGucgcgguCGUCCUCGa -3' miRNA: 3'- -GUCU-GCGuUGGGGUCUAU-------GCAGGGGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 138114 | 0.75 | 0.61463 |
Target: 5'- uUAGACGCcacGGCCCCacccAGAgACGUCCCCc -3' miRNA: 3'- -GUCUGCG---UUGGGG----UCUaUGCAGGGGu -5' |
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765 | 3' | -56 | NC_000852.3 | + | 293972 | 0.89 | 0.122725 |
Target: 5'- cCAGuCGCAAUCCCAGAcACGUCCCCAg -3' miRNA: 3'- -GUCuGCGUUGGGGUCUaUGCAGGGGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 293924 | 0.93 | 0.065319 |
Target: 5'- cCAGACGCAACCCCAGAcACGcCCCCAu -3' miRNA: 3'- -GUCUGCGUUGGGGUCUaUGCaGGGGU- -5' |
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765 | 3' | -56 | NC_000852.3 | + | 294020 | 1.08 | 0.007093 |
Target: 5'- cCAGACGCAACCCCAGAUACGUCCCCAg -3' miRNA: 3'- -GUCUGCGUUGGGGUCUAUGCAGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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