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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7650 | 3' | -51 | NC_001973.1 | + | 117998 | 0.66 | 0.993482 |
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Target: 5'- cGAGGAGACggGCUGCGuguUCG-AGAg-- -3' miRNA: 3'- uCUCCUUUG--CGACGCu--AGCaUCUgau -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 15400 | 0.66 | 0.992475 |
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Target: 5'- gGGAGGcGACGUucaauuccgcgaUGCGGUCGUgcAGAUc- -3' miRNA: 3'- -UCUCCuUUGCG------------ACGCUAGCA--UCUGau -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 117601 | 0.67 | 0.979318 |
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Target: 5'- aAGAGGAcuCGUccugGCGAggguucgaCGUAGACUGc -3' miRNA: 3'- -UCUCCUuuGCGa---CGCUa-------GCAUCUGAU- -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 43723 | 0.68 | 0.968392 |
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Target: 5'- uGAGGAAGCGCUGCucGAU-GgcGACc- -3' miRNA: 3'- uCUCCUUUGCGACG--CUAgCauCUGau -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 49954 | 0.69 | 0.961611 |
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Target: 5'- cGGAGGAGGCGCaGCuguUCGaGGACa- -3' miRNA: 3'- -UCUCCUUUGCGaCGcu-AGCaUCUGau -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 64743 | 0.69 | 0.953882 |
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Target: 5'- aGGAGGAcGCGCUGgaguCGAUCGguuGCUAu -3' miRNA: 3'- -UCUCCUuUGCGAC----GCUAGCaucUGAU- -5' |
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| 7650 | 3' | -51 | NC_001973.1 | + | 150759 | 1.07 | 0.010804 |
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Target: 5'- cAGAGGAAACGCUGCGAUCGUAGACUAu -3' miRNA: 3'- -UCUCCUUUGCGACGCUAGCAUCUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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