Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7651 | 5' | -59.6 | NC_001973.1 | + | 62570 | 0.66 | 0.786133 |
Target: 5'- uGUgcGAGGGCUcgaGCGGGcGCagcgacgcGUCGCUCg -3' miRNA: 3'- gCA--UUUCCGG---CGCCC-CGga------CAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 135667 | 0.66 | 0.77712 |
Target: 5'- gGUGuuGGCCGUGGcgcuGGCCUG-CGgUUu -3' miRNA: 3'- gCAUuuCCGGCGCC----CCGGACaGCgAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 78623 | 0.66 | 0.767984 |
Target: 5'- aCGacGAGGCCGCGGGcGCUcGagGCg- -3' miRNA: 3'- -GCauUUCCGGCGCCC-CGGaCagCGag -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 21272 | 0.66 | 0.767984 |
Target: 5'- -----cGaGCCGCGgcguccgccGGGCC-GUCGCUCa -3' miRNA: 3'- gcauuuC-CGGCGC---------CCCGGaCAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 83653 | 0.67 | 0.72075 |
Target: 5'- --cGAAGcGCUGCGGcGGCCcGcUCGCUUc -3' miRNA: 3'- gcaUUUC-CGGCGCC-CCGGaC-AGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 27872 | 0.67 | 0.72075 |
Target: 5'- -----cGGCCGCGGGcGCggGcgCGCUCu -3' miRNA: 3'- gcauuuCCGGCGCCC-CGgaCa-GCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 50345 | 0.67 | 0.72075 |
Target: 5'- uGUGAGGGCgaucgcucaaUGCGGGcaCCacgcgGUCGCUCa -3' miRNA: 3'- gCAUUUCCG----------GCGCCCc-GGa----CAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 132444 | 0.67 | 0.711052 |
Target: 5'- gGUGGAGGCgccgGCGGGGCgCggccucGUCggGCUCg -3' miRNA: 3'- gCAUUUCCGg---CGCCCCG-Ga-----CAG--CGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 21559 | 0.67 | 0.701291 |
Target: 5'- cCGUGGucccGGCgCGCGGGGUUgaagUGCUCg -3' miRNA: 3'- -GCAUUu---CCG-GCGCCCCGGaca-GCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 76052 | 0.67 | 0.691474 |
Target: 5'- ----cAGaCCGUcuucGuGGGCCUGUCGCUCg -3' miRNA: 3'- gcauuUCcGGCG----C-CCCGGACAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 81040 | 0.67 | 0.68161 |
Target: 5'- gGUAugcGGGCCGCGucgacGGGCgUG-CGCUUc -3' miRNA: 3'- gCAUu--UCCGGCGC-----CCCGgACaGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 17255 | 0.68 | 0.631869 |
Target: 5'- uGUAcucGGCCGCGGcGGCCg--CGCa- -3' miRNA: 3'- gCAUuu-CCGGCGCC-CCGGacaGCGag -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 92015 | 0.68 | 0.621891 |
Target: 5'- -----uGGCCGCaaucGCCUGUCGCUCc -3' miRNA: 3'- gcauuuCCGGCGccc-CGGACAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 61118 | 0.72 | 0.440594 |
Target: 5'- -----cGGCgGCGGGGCgCggcGUCGCUCc -3' miRNA: 3'- gcauuuCCGgCGCCCCG-Ga--CAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 52845 | 0.73 | 0.365583 |
Target: 5'- uGUAGAcGGCCGCGcGcGGCCgGUCGgUCc -3' miRNA: 3'- gCAUUU-CCGGCGC-C-CCGGaCAGCgAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 100091 | 0.73 | 0.35781 |
Target: 5'- aGUuucuGGUgGCGGGGUC-GUCGCUCg -3' miRNA: 3'- gCAuuu-CCGgCGCCCCGGaCAGCGAG- -5' |
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7651 | 5' | -59.6 | NC_001973.1 | + | 149307 | 1.09 | 0.001407 |
Target: 5'- cCGUAAAGGCCGCGGGGCCUGUCGCUCu -3' miRNA: 3'- -GCAUUUCCGGCGCCCCGGACAGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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