Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7652 | 3' | -53.2 | NC_001973.1 | + | 59114 | 0.66 | 0.972499 |
Target: 5'- aGCUgAGcgcGUUCGUGgCCAaGUUGGCg -3' miRNA: 3'- -CGAgUCuaaCGAGCACaGGU-CGACCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 100775 | 0.66 | 0.963131 |
Target: 5'- aCUCGGAggGCagcgcgcacaCGUGgCCAGCgGGCg -3' miRNA: 3'- cGAGUCUaaCGa---------GCACaGGUCGaCCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 10486 | 0.66 | 0.962085 |
Target: 5'- uGCUgGGGUUgcacgagcgggcgcGCUCGcgGUUCAGCgccaGGCa -3' miRNA: 3'- -CGAgUCUAA--------------CGAGCa-CAGGUCGa---CCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 51932 | 0.67 | 0.959566 |
Target: 5'- aGCcgCGGAggaCUCG-GUCUAGCUGGa -3' miRNA: 3'- -CGa-GUCUaacGAGCaCAGGUCGACCg -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 50524 | 0.68 | 0.933197 |
Target: 5'- cGCUCcuuGAa--CUCGg--CCAGCUGGCg -3' miRNA: 3'- -CGAGu--CUaacGAGCacaGGUCGACCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 91933 | 0.68 | 0.922444 |
Target: 5'- aGCa-AGAgUUGUUCGUGUugcgCCAGCUuGGCg -3' miRNA: 3'- -CGagUCU-AACGAGCACA----GGUCGA-CCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 120066 | 0.68 | 0.910699 |
Target: 5'- cGUUgAGGgugcGCUCGUcGUCCAGCUGuuuGCc -3' miRNA: 3'- -CGAgUCUaa--CGAGCA-CAGGUCGAC---CG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 10332 | 0.69 | 0.904456 |
Target: 5'- cGCUCguugAGGUcgGCgaugCGgccGUUCGGCUGGCa -3' miRNA: 3'- -CGAG----UCUAa-CGa---GCa--CAGGUCGACCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 101134 | 0.69 | 0.897971 |
Target: 5'- --gCAGAUaGCUCGUGUUgGaGCUGaGCa -3' miRNA: 3'- cgaGUCUAaCGAGCACAGgU-CGAC-CG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 32002 | 0.69 | 0.884283 |
Target: 5'- cGCUCAG---GC-CGUGcaaCAGCUGGCc -3' miRNA: 3'- -CGAGUCuaaCGaGCACag-GUCGACCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 52855 | 0.72 | 0.774961 |
Target: 5'- cGCgcgCGGcc-GgUCG-GUCCAGCUGGCg -3' miRNA: 3'- -CGa--GUCuaaCgAGCaCAGGUCGACCG- -5' |
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7652 | 3' | -53.2 | NC_001973.1 | + | 146559 | 1.15 | 0.002066 |
Target: 5'- cGCUCAGAUUGCUCGUGUCCAGCUGGCa -3' miRNA: 3'- -CGAGUCUAACGAGCACAGGUCGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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