Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7654 | 5' | -58.7 | NC_001973.1 | + | 58773 | 0.7 | 0.574512 |
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Target: 5'- aGUGC-CG-GCGC-GCCGUGGugaACCUGAg -3' miRNA: 3'- -CAUGuGCuCGCGcCGGCACC---UGGACU- -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 150045 | 0.71 | 0.516081 |
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Target: 5'- -gGC-CGGGCGCGGCCGaaggcUGGAaaUCUGGg -3' miRNA: 3'- caUGuGCUCGCGCCGGC-----ACCU--GGACU- -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 53513 | 0.72 | 0.49712 |
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Target: 5'- uGUACACGGcCGC-GCCGUcGGGCCUGu -3' miRNA: 3'- -CAUGUGCUcGCGcCGGCA-CCUGGACu -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 136436 | 0.72 | 0.478479 |
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Target: 5'- cUGCGCGAGCGCaggGGCgCGUaGGGCCg-- -3' miRNA: 3'- cAUGUGCUCGCG---CCG-GCA-CCUGGacu -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 76575 | 0.74 | 0.354435 |
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Target: 5'- -aACGaauCGAGCGUGGCCGaggcggccaagcaguUGGGCCUGGc -3' miRNA: 3'- caUGU---GCUCGCGCCGGC---------------ACCUGGACU- -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 45829 | 0.84 | 0.092415 |
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Target: 5'- uGUGCGCcGGCGCGGCguuCGUGGGCCUGGg -3' miRNA: 3'- -CAUGUGcUCGCGCCG---GCACCUGGACU- -5' |
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| 7654 | 5' | -58.7 | NC_001973.1 | + | 145587 | 1.08 | 0.001981 |
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Target: 5'- uGUACACGAGCGCGGCCGUGGACCUGAg -3' miRNA: 3'- -CAUGUGCUCGCGCCGGCACCUGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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