Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7659 | 3' | -52.7 | NC_001973.1 | + | 35823 | 0.66 | 0.97061 |
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Target: 5'- -cGuACACGCucaUGggGCcGCUGUGg -3' miRNA: 3'- gaCuUGUGCGuggACuuCGuCGACAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 28930 | 0.7 | 0.877944 |
|
Target: 5'- uUGGgcuuGCACGCGgCUGggGCGGCa--- -3' miRNA: 3'- gACU----UGUGCGUgGACuuCGUCGacac -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 23214 | 0.69 | 0.89899 |
|
Target: 5'- -cGAGCGCGCaguggcgcaggGCCUGgcGCuGCUGa- -3' miRNA: 3'- gaCUUGUGCG-----------UGGACuuCGuCGACac -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 16136 | 0.73 | 0.714703 |
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Target: 5'- -cGAGCACGCGCCgcuacAGCAuucgucGCUGUGg -3' miRNA: 3'- gaCUUGUGCGUGGacu--UCGU------CGACAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 11276 | 0.7 | 0.846646 |
|
Target: 5'- -cGAGCGCGCACac--GGCcaAGCUGUGg -3' miRNA: 3'- gaCUUGUGCGUGgacuUCG--UCGACAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 1825 | 0.7 | 0.846646 |
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Target: 5'- -cGAGCGCGCACac--GGCcaGGCUGUGg -3' miRNA: 3'- gaCUUGUGCGUGgacuUCG--UCGACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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