Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 7663 | 3' | -55.6 | NC_001973.1 | + | 146013 | 0.72 | 0.667763 |
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Target: 5'- gCGGGuCCGCCGgcucgagcGCGggUCgACGGACg -3' miRNA: 3'- aGCUC-GGCGGCaa------CGCuaAG-UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 93746 | 0.73 | 0.616903 |
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Target: 5'- gCGAGCCGCCcg-GCGAccaggaGCGGGCc -3' miRNA: 3'- aGCUCGGCGGcaaCGCUaag---UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 5747 | 0.73 | 0.586461 |
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Target: 5'- aCGGGCCGCCGaaGCGGccCACGGcGCu -3' miRNA: 3'- aGCUCGGCGGCaaCGCUaaGUGCC-UG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 152311 | 0.74 | 0.536459 |
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Target: 5'- aUCGAGCgCGCuCGUUaucguggacGCGGUgCGCGGGCg -3' miRNA: 3'- -AGCUCG-GCG-GCAA---------CGCUAaGUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 35836 | 0.75 | 0.488022 |
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Target: 5'- -gGGGCCGCUGUggGCGAUcCggACGGACa -3' miRNA: 3'- agCUCGGCGGCAa-CGCUAaG--UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 18625 | 0.76 | 0.441742 |
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Target: 5'- aCGAGCCGCCGUU-CGAgccgUCcgacggcccgACGGGCa -3' miRNA: 3'- aGCUCGGCGGCAAcGCUa---AG----------UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 32566 | 0.78 | 0.348653 |
|
Target: 5'- cCGAcGCCGCCGUcggcucggcgcgcUGCGA--CACGGACg -3' miRNA: 3'- aGCU-CGGCGGCA-------------ACGCUaaGUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 30362 | 0.79 | 0.298242 |
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Target: 5'- uUCGGGCCGCCGgcgcgGCGAcUCggguGCGGGCc -3' miRNA: 3'- -AGCUCGGCGGCaa---CGCUaAG----UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 139818 | 1.11 | 0.002634 |
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Target: 5'- aUCGAGCCGCCGUUGCGAUUCACGGACg -3' miRNA: 3'- -AGCUCGGCGGCAACGCUAAGUGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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