Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7663 | 3' | -55.6 | NC_001973.1 | + | 125677 | 0.68 | 0.868143 |
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Target: 5'- aCGuGCacgGCgGUUGCGGU-CACGGAa -3' miRNA: 3'- aGCuCGg--CGgCAACGCUAaGUGCCUg -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 132533 | 0.66 | 0.925673 |
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Target: 5'- -aGAgGCCGCCGggcGCGGcg-GCGGGCu -3' miRNA: 3'- agCU-CGGCGGCaa-CGCUaagUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 134992 | 0.69 | 0.793863 |
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Target: 5'- cCGGGCCGUCGg-GCGGgagC-CGGGCa -3' miRNA: 3'- aGCUCGGCGGCaaCGCUaa-GuGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 139818 | 1.11 | 0.002634 |
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Target: 5'- aUCGAGCCGCCGUUGCGAUUCACGGACg -3' miRNA: 3'- -AGCUCGGCGGCAACGCUAAGUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 140526 | 0.72 | 0.637267 |
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Target: 5'- -aGAGCaCGCUGcUGCGAUUCAUcGACu -3' miRNA: 3'- agCUCG-GCGGCaACGCUAAGUGcCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 146013 | 0.72 | 0.667763 |
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Target: 5'- gCGGGuCCGCCGgcucgagcGCGggUCgACGGACg -3' miRNA: 3'- aGCUC-GGCGGCaa------CGCuaAG-UGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 152311 | 0.74 | 0.536459 |
|
Target: 5'- aUCGAGCgCGCuCGUUaucguggacGCGGUgCGCGGGCg -3' miRNA: 3'- -AGCUCG-GCG-GCAA---------CGCUAaGUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 152375 | 0.69 | 0.793863 |
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Target: 5'- gCGAGCCggacgGCCGgcGCGG---GCGGGCg -3' miRNA: 3'- aGCUCGG-----CGGCaaCGCUaagUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 155106 | 0.71 | 0.707981 |
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Target: 5'- --aAGCCGCCGUcGCuucuaUCACGGGCg -3' miRNA: 3'- agcUCGGCGGCAaCGcua--AGUGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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