Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7663 | 3' | -55.6 | NC_001973.1 | + | 88485 | 0.67 | 0.875432 |
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Target: 5'- aCGGuGCCGCCGgcGCGg--CGCGG-Cu -3' miRNA: 3'- aGCU-CGGCGGCaaCGCuaaGUGCCuG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 8845 | 0.67 | 0.882507 |
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Target: 5'- cCGcGCUGCUGgcGCGGcgaCACGGGCu -3' miRNA: 3'- aGCuCGGCGGCaaCGCUaa-GUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 21549 | 0.67 | 0.882507 |
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Target: 5'- gUCGGGCgCGCCGUgguccCGGcgCGCGGGg -3' miRNA: 3'- -AGCUCG-GCGGCAac---GCUaaGUGCCUg -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 51472 | 0.67 | 0.889361 |
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Target: 5'- cUCGAGCgGCCGgguCGAUgcgCuCGGAUg -3' miRNA: 3'- -AGCUCGgCGGCaacGCUAa--GuGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 78137 | 0.67 | 0.889361 |
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Target: 5'- aCGuGCaCGaCCGggGCcuGAUUCGCGGAg -3' miRNA: 3'- aGCuCG-GC-GGCaaCG--CUAAGUGCCUg -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 38837 | 0.67 | 0.902393 |
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Target: 5'- -gGAGgCGgCGgcgGCGAgggCGCGGGCg -3' miRNA: 3'- agCUCgGCgGCaa-CGCUaa-GUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 36340 | 0.67 | 0.906738 |
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Target: 5'- gCGGGCCucGgCGUucaagagcaauaccUGCGGUaCGCGGACu -3' miRNA: 3'- aGCUCGG--CgGCA--------------ACGCUAaGUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 58643 | 0.67 | 0.908565 |
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Target: 5'- uUCGAcGCCGgCGgcGUGuucaaCGCGGACg -3' miRNA: 3'- -AGCU-CGGCgGCaaCGCuaa--GUGCCUG- -5' |
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| 7663 | 3' | -55.6 | NC_001973.1 | + | 22735 | 0.66 | 0.940654 |
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Target: 5'- gCGAGCCgcgGCCGUaucugUGCGAg--GgGGGCg -3' miRNA: 3'- aGCUCGG---CGGCA-----ACGCUaagUgCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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