miRNA: 3'- aGUCUACGa-------GGGCUGUGgGCCG-GUGG- -5'
miRNA: 3'- aGUCua-CGAGG---GCUGUGggCCGGUGg -5'
miRNA: 3'- -AGUCU---ACGa----GGGCUg---------UG-GGCCGGUGg -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7666 | 3' | -59 | NC_001973.1 | + | 7255 | 0.74 | 0.359785 |
|
Target: 5'- gCAGAUGCgcagcgggCUCGGCGCgCGGCgCGCCc -3' miRNA: 3'- aGUCUACGa-------GGGCUGUGgGCCG-GUGG- -5' |
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| 7666 | 3' | -59 | NC_001973.1 | + | 3485 | 0.71 | 0.534884 |
|
Target: 5'- aCGGucaGCUCCacgCGGCGCuuGGCCACg -3' miRNA: 3'- aGUCua-CGAGG---GCUGUGggCCGGUGg -5' |
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| 7666 | 3' | -59 | NC_001973.1 | + | 2593 | 0.68 | 0.672843 |
|
Target: 5'- uUCAGAcuuUGCgaccgCCCGAagaaaacuaaGCgCCGGCCGCg -3' miRNA: 3'- -AGUCU---ACGa----GGGCUg---------UG-GGCCGGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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