Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7668 | 3' | -48 | NC_001973.1 | + | 124272 | 0.66 | 0.999619 |
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Target: 5'- -cGugGGCCCGUCGGccgacgcuCUGAUg -3' miRNA: 3'- guCugUCGGGUAGUCuuuuu---GACUGu -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 76593 | 0.66 | 0.999427 |
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Target: 5'- gAGGCGGCCaagCAGuuGGGcCUGGCGg -3' miRNA: 3'- gUCUGUCGGguaGUCuuUUU-GACUGU- -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 111086 | 0.66 | 0.999119 |
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Target: 5'- -cGugGGCCgCGUCgAGGAGAGC-GACGa -3' miRNA: 3'- guCugUCGG-GUAG-UCUUUUUGaCUGU- -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 110618 | 0.66 | 0.999119 |
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Target: 5'- aGGACAuGCUCAUgCAGAAGAGC-GAUu -3' miRNA: 3'- gUCUGU-CGGGUA-GUCUUUUUGaCUGu -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 83789 | 0.67 | 0.998679 |
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Target: 5'- uCGGucGCGGCCCGgcacUCGGAcGAGCUGGg- -3' miRNA: 3'- -GUC--UGUCGGGU----AGUCUuUUUGACUgu -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 55948 | 0.67 | 0.998397 |
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Target: 5'- aCGGACucgGGCCCGUUcugucgGGAGAGGCgcgcGACGa -3' miRNA: 3'- -GUCUG---UCGGGUAG------UCUUUUUGa---CUGU- -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 73714 | 0.67 | 0.998397 |
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Target: 5'- aCGGGC-GCgCCGUCGuAAAAGCUGAUg -3' miRNA: 3'- -GUCUGuCG-GGUAGUcUUUUUGACUGu -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 52859 | 0.67 | 0.997677 |
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Target: 5'- --cGCGGCCgGUCGGuccAGCUGGCGc -3' miRNA: 3'- gucUGUCGGgUAGUCuuuUUGACUGU- -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 144983 | 0.74 | 0.897631 |
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Target: 5'- -cGGCAGCCCAUCgucgAGGAGGACuucuUGGCGg -3' miRNA: 3'- guCUGUCGGGUAG----UCUUUUUG----ACUGU- -5' |
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| 7668 | 3' | -48 | NC_001973.1 | + | 137946 | 1.09 | 0.015056 |
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Target: 5'- cCAGACAGCCCAUCAGAAAAACUGACAu -3' miRNA: 3'- -GUCUGUCGGGUAGUCUUUUUGACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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