Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7669 | 3' | -55.9 | NC_001973.1 | + | 94289 | 0.66 | 0.90446 |
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Target: 5'- -cGCCGAgggaAGGCggCgcgCCCGCCAUc- -3' miRNA: 3'- uuCGGCUag--UCCGa-Ga--GGGUGGUAac -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 2720 | 0.66 | 0.898004 |
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Target: 5'- gGGGUCGAUCGGGUUCaagCCCgaugagaucacGCCAa-- -3' miRNA: 3'- -UUCGGCUAGUCCGAGa--GGG-----------UGGUaac -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 126474 | 0.67 | 0.829739 |
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Target: 5'- cAGCCGGUUgAGGCUCUgCCUAaaGUUGc -3' miRNA: 3'- uUCGGCUAG-UCCGAGA-GGGUggUAAC- -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 33122 | 0.68 | 0.803401 |
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Target: 5'- cAGGUCGAUCAGGCgcugCgucgcgCCCugCAg-- -3' miRNA: 3'- -UUCGGCUAGUCCGa---Ga-----GGGugGUaac -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 98362 | 0.69 | 0.766092 |
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Target: 5'- cGGCCGcuuUCGGGCUUUgCCUACCGc-- -3' miRNA: 3'- uUCGGCu--AGUCCGAGA-GGGUGGUaac -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 123227 | 0.69 | 0.716768 |
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Target: 5'- uGGUCG-UCcGGCUCggcgUCCCGCCGUUGc -3' miRNA: 3'- uUCGGCuAGuCCGAG----AGGGUGGUAAC- -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 112658 | 0.73 | 0.531745 |
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Target: 5'- cAAGCCGGUCAGcCUCUUCagcugGCCGUUGg -3' miRNA: 3'- -UUCGGCUAGUCcGAGAGGg----UGGUAAC- -5' |
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| 7669 | 3' | -55.9 | NC_001973.1 | + | 133625 | 1.05 | 0.004433 |
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Target: 5'- gAAGCCGAUCAGGCUCUCCCACCAUUGc -3' miRNA: 3'- -UUCGGCUAGUCCGAGAGGGUGGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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