Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7672 | 3' | -56.8 | NC_001973.1 | + | 28979 | 0.68 | 0.806882 |
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Target: 5'- cGACGCGUuguagUCGGggaagGGcGCCGGcggCUGGg -3' miRNA: 3'- uCUGCGCG-----AGCCa----CCuUGGCCa--GAUC- -5' |
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| 7672 | 3' | -56.8 | NC_001973.1 | + | 3616 | 0.68 | 0.806882 |
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Target: 5'- -cACGCGCgCGGUGGuGuuGGUCa-- -3' miRNA: 3'- ucUGCGCGaGCCACCuUggCCAGauc -5' |
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| 7672 | 3' | -56.8 | NC_001973.1 | + | 44609 | 0.69 | 0.752182 |
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Target: 5'- -aGCGgGCUCGGcccGACCGGUCUAu -3' miRNA: 3'- ucUGCgCGAGCCaccUUGGCCAGAUc -5' |
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| 7672 | 3' | -56.8 | NC_001973.1 | + | 121558 | 0.69 | 0.742655 |
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Target: 5'- cGGCGCGUgUGGUGGAACacguacucguCGGUCg-- -3' miRNA: 3'- uCUGCGCGaGCCACCUUG----------GCCAGauc -5' |
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| 7672 | 3' | -56.8 | NC_001973.1 | + | 61199 | 0.7 | 0.693792 |
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Target: 5'- -cGCGCGCUCGGgcgGGcGCCGGcgCUc- -3' miRNA: 3'- ucUGCGCGAGCCa--CCuUGGCCa-GAuc -5' |
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| 7672 | 3' | -56.8 | NC_001973.1 | + | 131887 | 1.08 | 0.003441 |
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Target: 5'- cAGACGCGCUCGGUGGAACCGGUCUAGc -3' miRNA: 3'- -UCUGCGCGAGCCACCUUGGCCAGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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