Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7673 | 3' | -56.1 | NC_001973.1 | + | 14333 | 0.73 | 0.533484 |
Target: 5'- cGAGUGCAaccGGCCGCACuugGCGUGGUcgGCg -3' miRNA: 3'- -UUCGCGU---CUGGCGUG---UGCACCAcaUG- -5' |
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7673 | 3' | -56.1 | NC_001973.1 | + | 3732 | 0.73 | 0.533484 |
Target: 5'- -cGUGCAGGCCGUACACGccgacGGgcacGUGCg -3' miRNA: 3'- uuCGCGUCUGGCGUGUGCa----CCa---CAUG- -5' |
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7673 | 3' | -56.1 | NC_001973.1 | + | 31870 | 0.74 | 0.465392 |
Target: 5'- uAGGCGguGG-CGCGCACGUcGGUGUGg -3' miRNA: 3'- -UUCGCguCUgGCGUGUGCA-CCACAUg -5' |
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7673 | 3' | -56.1 | NC_001973.1 | + | 131330 | 1.08 | 0.002907 |
Target: 5'- gAAGCGCAGACCGCACACGUGGUGUACg -3' miRNA: 3'- -UUCGCGUCUGGCGUGUGCACCACAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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