Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 768 | 3' | -57.9 | NC_000852.3 | + | 101083 | 0.66 | 0.954855 |
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Target: 5'- uGCCGaCGUcuuucaGCAGCACGUGUGaauuUUGUUACu -3' miRNA: 3'- -CGGC-GCA------UGUCGUGCGCGU----AGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 306587 | 0.66 | 0.947121 |
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Target: 5'- -gCGCGUGCuGUACGCGgCAUuCGCg-- -3' miRNA: 3'- cgGCGCAUGuCGUGCGC-GUA-GCGgug -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 263423 | 0.67 | 0.933966 |
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Target: 5'- uCCGCGUAgAGaucuuuuucCGCGUccguuucaaaAUCGCCACg -3' miRNA: 3'- cGGCGCAUgUCgu-------GCGCG----------UAGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 251806 | 0.67 | 0.907817 |
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Target: 5'- cGCCGCa-GCAGCucCGUGCGcCGUCAUg -3' miRNA: 3'- -CGGCGcaUGUCGu-GCGCGUaGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 308407 | 0.68 | 0.895889 |
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Target: 5'- uCCGCGUAgAGCAgGUGaucgucUCGCUGCa -3' miRNA: 3'- cGGCGCAUgUCGUgCGCgu----AGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 228853 | 0.69 | 0.832565 |
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Target: 5'- uGCCGcCGUAUguguuGGUACauGCGCuguauAUCGCCGCg -3' miRNA: 3'- -CGGC-GCAUG-----UCGUG--CGCG-----UAGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 246441 | 0.71 | 0.765262 |
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Target: 5'- gGCCaGCGUGCcguGGaCAUGCGCgAUCGUCAUc -3' miRNA: 3'- -CGG-CGCAUG---UC-GUGCGCG-UAGCGGUG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 95990 | 0.72 | 0.710182 |
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Target: 5'- cGCCGCGUAU-GC-CGCGauaAUCGCC-Cg -3' miRNA: 3'- -CGGCGCAUGuCGuGCGCg--UAGCGGuG- -5' |
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| 768 | 3' | -57.9 | NC_000852.3 | + | 306935 | 1.11 | 0.00299 |
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Target: 5'- uGCCGCGUACAGCACGCGCAUCGCCACa -3' miRNA: 3'- -CGGCGCAUGUCGUGCGCGUAGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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