Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
769 | 3' | -50.2 | NC_000852.3 | + | 164361 | 0.66 | 0.999766 |
Target: 5'- cGAAGAUggaacGAUGGGACGUAuGGUCg-- -3' miRNA: 3'- aCUUCUGa----CUGCUCUGUAU-CCGGaac -5' |
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769 | 3' | -50.2 | NC_000852.3 | + | 179639 | 0.68 | 0.998693 |
Target: 5'- aGAAGACUcaugaGGCGAGACAUGacuggggggguuccGGCUg-- -3' miRNA: 3'- aCUUCUGA-----CUGCUCUGUAU--------------CCGGaac -5' |
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769 | 3' | -50.2 | NC_000852.3 | + | 249219 | 0.73 | 0.964815 |
Target: 5'- gGggGACaagUGACGAGACAgcGGUCg-- -3' miRNA: 3'- aCuuCUG---ACUGCUCUGUauCCGGaac -5' |
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769 | 3' | -50.2 | NC_000852.3 | + | 310302 | 1.09 | 0.021874 |
Target: 5'- cUGAAGACUGACGAGACAUAGGCCUUGu -3' miRNA: 3'- -ACUUCUGACUGCUCUGUAUCCGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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