Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7691 | 5' | -46.2 | NC_001973.1 | + | 94919 | 0.66 | 0.999974 |
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Target: 5'- --gGCAAGGUgguggcCGCCUaCUUCGucGAGu -3' miRNA: 3'- acaCGUUCUAa-----GCGGA-GAAGCuuUUC- -5' |
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| 7691 | 5' | -46.2 | NC_001973.1 | + | 126857 | 0.67 | 0.999888 |
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Target: 5'- cGUGCAcagcaccugcGGAaUCaCCUCUUCGAGu-- -3' miRNA: 3'- aCACGU----------UCUaAGcGGAGAAGCUUuuc -5' |
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| 7691 | 5' | -46.2 | NC_001973.1 | + | 46567 | 0.67 | 0.999854 |
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Target: 5'- cGUGCucgAGGAcaCGCCUCUgcaUCGAAu-- -3' miRNA: 3'- aCACG---UUCUaaGCGGAGA---AGCUUuuc -5' |
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| 7691 | 5' | -46.2 | NC_001973.1 | + | 83451 | 0.67 | 0.999811 |
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Target: 5'- gGUGCGAcag-CGCCgcgCUcgUCGAGGAGg -3' miRNA: 3'- aCACGUUcuaaGCGGa--GA--AGCUUUUC- -5' |
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| 7691 | 5' | -46.2 | NC_001973.1 | + | 38040 | 0.68 | 0.999502 |
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Target: 5'- cGUuuGAGAUUCGUCgUCUUCGcuGGGg -3' miRNA: 3'- aCAcgUUCUAAGCGG-AGAAGCuuUUC- -5' |
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| 7691 | 5' | -46.2 | NC_001973.1 | + | 118904 | 1.11 | 0.020272 |
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Target: 5'- cUGUGCAAGAUUCGCCUCUUCGAAAAGa -3' miRNA: 3'- -ACACGUUCUAAGCGGAGAAGCUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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